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Electrospun biosystems made of nylon 6 and laccase and its application in dyes removal
Environmental Technology & Innovation ( IF 6.7 ) Pub Date : 2020-12-28 , DOI: 10.1016/j.eti.2020.101332
Katarzyna Jankowska , Adam Grzywaczyk , Adam Piasecki , Ewa Kijeńska-Gawrońska , Luong N. Nguyen , Jakub Zdarta , Long D. Nghiem , Manuel Pinelo , Teofil Jesionowski

Electrospun materials, due to the possibility of design of their properties, are suitable as supports for enzyme immobilization. Produced biocatalytic systems might be then apply in various biocatalytic reactions, including conversion of pollutants. In our study, electrospun fibers made from nylon 6 was produced, modified and applied as a support for laccase immobilization by adsorption and covalent binding. The systems with immobilized laccase were used in decolorization process of selected dyes, azo dye Reactive Black 5 and the anthraquinone dye Reactive Blue 4. It was found that at from dye solution at concentration 1 mg/L at pH 5, temperature 25 °C, after 24 h of process the efficiency of decolorization of Reactive Blue 4 and Reactive Black 5 reached 77% and 63%, respectively. The storage stability studies showed that after 30 days of storage, the relative activities were 60% and 95% for adsorbed and covalently bonded oxidoreductase respectively. Moreover, after 10 consecutive catalytic cycles adsorbed and covalently bonded laccase retained over 60% and 70% respectively, indicating the possibility of application of the obtained systems on a larger scale for removal of phenolic pollutants from wastewaters.



中文翻译:

尼龙6和漆酶组成的电纺生物系统及其在染料去除中的应用。

由于电纺材料具有设计其特性的可能性,因此适合作为酶固定的载体。然后,产生的生物催化系统可能会应用于各种生物催化反应,包括污染物的转化。在我们的研究中,尼龙6制成的电纺纤维被生产,改性并通过吸附和共价键合作为漆酶固定化的载体。固定有漆酶的体系用于所选染料,偶氮染料Reactive Black 5和蒽醌染料Reactive Blue 4的脱色过程中。发现在pH 5,温度25°C,浓度为1 mg / L的染料溶液中,在处理24小时后,活性蓝4和活性黑5的脱色效率分别达到77%和63%。储存稳定性研究表明,储存30天后,吸附和共价键氧化还原酶的相对活性分别为60%和95%。此外,在连续10次催化循环后,吸附的和共价键结合的漆酶分别保留了60%和70%以上,这表明有可能将所获得的系统大规模应用于从废水中去除酚类污染物。

更新日期:2020-12-28
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