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Solid-phase microextraction integrated nanobiosensors for the serial detection of cytoplasmic dopamine in a single living cell
Biosensors and Bioelectronics ( IF 10.7 ) Pub Date : 2020-12-28 , DOI: 10.1016/j.bios.2020.112915
Yaran Chang , Yongjia Chen , Yunlong Shao , Boye Li , Yuanyuan Wu , Wenmei Zhang , Yingyan Zhou , Zhihui Yu , Liping Lu , Xiayan Wang , Guangsheng Guo

Dopamine participates in many physiological and pathological processes. Dynamic monitoring of dopamine levels in the cytoplasm of a single living cell reflects not only the functional state of dopamine synthesis factors but also the processes of related neurodegenerative diseases. Due to the low content of cytoplasmic dopamine and the difficulty to keep cells alive during the operating process, the detection of cytoplasmic dopamine is still challenging. Herein, a solid-phase microextraction (SPME) technique integrated nanobiosensor was employed to trace and quantify dopamine concentration fluctuations in the cytoplasm of a single living cell. We designed a polypyrrole modified carbon fiber nanoprobe as a bifunctional nanoprobe that can extract cytoplasmic dopamine and then perform electrochemical detection. This bifunctional nanoprobe can detect 10 pmol/L extracted dopamine and detected a 60% decrease of the cytoplasmic dopamine concentration in a single living cell by K+ stimulation. This study allowed for the first time serially detecting cytoplasmic dopamine while keeping the target cell alive, which might yield a new method for research on dopamine neurotoxicity and the related drug action mechanisms for neurodegenerative disease.



中文翻译:

-固相微萃取集成细胞质多巴胺的序列检测纳米生物传感器在单个活细胞

Dopamine participates in many physiological and pathological processes. Dynamic monitoring of dopamine levels in the cytoplasm of a single living cell reflects not only the functional state of dopamine synthesis factors but also the processes of related neurodegenerative diseases. Due to the low content of cytoplasmic dopamine and the difficulty to keep cells alive during the operating process, the detection of cytoplasmic dopamine is still challenging. Herein, a solid-phase microextraction (SPME) technique integrated nanobiosensor was employed to trace and quantify dopamine concentration fluctuations in the cytoplasm of a single living cell. We designed a polypyrrole modified carbon fiber nanoprobe as a bifunctional nanoprobe that can extract cytoplasmic dopamine and then perform electrochemical detection. This bifunctional nanoprobe can detect 10 pmol/L extracted dopamine and detected a 60% decrease of the cytoplasmic dopamine concentration in a single living cell by K+刺激。这项研究首次允许在保持靶细胞存活的同时连续检测细胞质多巴胺,这可能为研究多巴胺神经毒性和相关的神经退行性疾病的药物作用机制提供一种新方法。

更新日期:2020-12-28
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