Abstract

For the well-known theoretical model of hydrodynamic chromatography, which relates the relative retention of an analyte and the separation efficiency to the characteristic parameter of a system, expressions are proposed that make it possible to find the optimal conditions for analysis. The theoretical model well predicts the relative retention of analytes but greatly overestimates the separation efficiency, especially for high molecular weight polymers. The observed discrepancy is due to the impossibility within one separation to create optimal conditions for all components of the sample and/or the influence of the polydispersity of the studied polymer samples on the separation efficiency.

中文翻译：

---

水力色谱法优化聚合物分离条件的研究

摘要

对于众所周知的流体动力学色谱理论模型，该模型将分析物的相对保留率和分离效率与系统的特征参数相关联，提出了可以找到最佳分析条件的表达式。理论模型很好地预测了分析物的相对保留，但大大高估了分离效率，尤其是对于高分子量聚合物。观察到的差异是由于不可能在一次分离中为样品的所有组分创造最佳条件和/或所研究的聚合物样品的多分散性对分离效率的影响。