ABSTRACT

Spiromesifen (Oberon® 240 SC; concentrated solution; Bayer CropScience, Germany) a pesticide derived from spirocyclic tetronic acids, acts mainly though blocking lipid biosynthesis via acetyl-CoA carboxylase. However, its mode of action requires further analyses. This study aims to evaluate the sublethal effects of spiromesifen on the fruit fly Drosophila melanogaster. The treatment was carried out by topical application on newly moulted pupae using two concentrations (LD₁₀ = 21.45 and LD₂₅ = 39.53 μg a.i./pupa). Our results showed that the developmental duration of larvae and pupae were not significantly affected by treatment. However, spiromesifen caused delayed effects on flies that survived from treated pupae. Spiromesifen was found to decrease significantly both the morphometric measurements and the protein contents of ovaries. The protein pattern of ovaries showed a reduction in the number of protein bands affecting the progeny via vitellogenesis. Finally, compared to controls, spiromesifen decreased significantly the amounts of some cuticular hydrocarbons in treated female and male adults. Consequently, in response to the changes in cuticular hydrocarbons, their sexual behaviour was also affected. Our study brings new insights about the multiple effects and physiological consequences of disturbing lipid metabolism.

抽象的

Spiromesifen（Oberon®240 SC；浓溶液； Bayer CropScience，德国）是一种从螺环四氢代酸衍生的农药，其主要作用是通过乙酰辅酶A羧化酶阻断脂质的生物合成。但是，其作用方式需要进一步分析。本研究旨在评估螺美斯芬对果蝇果蝇的亚致死作用。通过使用两种浓度的局部蜕皮对新蜕皮的up进行处理（LD ₁₀  = 21.45和LD ₂₅ ＝ 39.53μgai / pupa）。我们的结果表明，幼虫和p的发育持续时间不受治疗的影响显着。然而，螺索米芬对从已处理的up存活下来的果蝇造成延迟的影响。发现Spiromesifen会显着降低卵巢的形态学测量值和蛋白质含量。卵巢的蛋白质模式显示通过卵黄发生影响后代的蛋白质条带数量减少。最后，与对照组相比，螺旋藻西芬显着降低了治疗的成年女性和成年男性中某些表皮碳氢化合物的含量。因此，响应于表皮碳氢化合物的变化，它们的性行为也受到影响。我们的研究为干扰脂质代谢的多种作用和生理后果带来了新的见解。