An 8‐week feeding experiment was performed to evaluate the effects of dietary arachidonic acid (ARA) on the reproductive performance, tissue fatty acid profile and sex hormone secretion in female Pelteobagrus fulvidraco during the breeding season. Three diets with different ARA levels (0.64%, 4.67% and 10.07% of total fatty acids) were formulated, a control diet (C) and two diets with low ARA (ARA‐L) or high ARA (ARA‐H). The results showed that the highest gonadosomatic index (GSI) and condition factor (CF) were observed in the ARA‐H group. The diets with supplementation of ARA resulted in a significantly higher fertilization rate, more normal larvae and greater survival rate of 7 days posthatching (7DPH) larvae. The ARA contents in liver and 3DPH larvae were improved with increasing dietary ARA concentrations. The lowest EPA/ARA ratios were observed in liver and eggs of fish fed with the ARA‐L diet. Significantly reduced serum estradiol (E2), 11‐keto‐testosterone (11‐KT) and testosterone (T) levels were found in the ARA‐L group females compared with the other treatments. The combined results indicated that a higher dietary ARA level and ARA/EPA ratio mainly influence spawning performance and egg quality of yellow catfish and that ARA regulates sex steroid hormone synthesis in broodstock.

中文翻译：

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日粮花生四烯酸对黄yellow鱼繁殖性能，组织脂肪酸谱和性腺类固醇生成的影响

进行了为期8周的喂养实验，以评估日粮花生四烯酸（ARA）对雌性小叶黄Pe（Pelteobagrus fulvidraco）生殖性能，组织脂肪酸谱和性激素分泌的影响在繁殖季节。配制了三种具有不同ARA水平的饮食（分别占总脂肪酸的0.64％，4.67％和10.07％），一种对照饮食（C）和两种具有低ARA（ARA-L）或高ARA（ARA-H）的饮食。结果表明，ARA-H组的促性腺激素指数（GSI）和条件因子（CF）最高。补充ARA的饮食可显着提高受精率，更正常的幼虫和孵化后7天（7DPH）幼虫的存活率。肝脏和3DPH幼虫中的ARA含量随着日粮中ARA浓度的增加而提高。饲喂ARA‐L日粮的鱼的肝脏和鸡蛋中EPA / ARA比率最低。血清雌二醇（E2）显着降低，与其他治疗方法相比，ARA‐L组女性中发现11-酮-睾酮（11-KT）和睾酮（T）的水平。综合结果表明，较高的日粮ARA水平和ARA / EPA比值主要影响黄cat鱼的产卵性能和卵品质，并且ARA调节亲鱼中性类固醇激素的合成。