A 10‐week feed trial was conducted to investigate the effects of tributyrin (TB) supplementation on growth performance, body composition, serum biochemical indexes and lipid metabolism‐related genes expression of juvenile large yellow croaker (Larimichthys crocea) fed diets containing high level of soybean oil. In the negative control group, 80 g/kg fish oil was used as the only lipid source (FO group), while in the positive control group, 20 g/kg fish oil and 60 g/kg soybean oil were used as the lipid source (FS group). Based on the positive control diet, grade levels of 1, 2, 4 and 8 g/kg tributyrin were added, respectively. Results showed that compared with the negative control group, high level of dietary soybean oil significantly decreased the growth performance and increased the liver lipids deposition of juvenile large yellow croaker. Compared with the positive control group, supplementation of 2 g/kg tributyrin significantly increased the growth performance and reduced the liver triglycerides and serum cholesterol content of juvenile large yellow croaker. Supplementation of 4 g/kg tributyrin significantly decreased liver triglycerides and serum cholesterol and free fatty acids content of juvenile large yellow croaker. Moreover, supplementation of 2 and 4 g/kg tributyrin significantly decreased the expression of acc, srebp‐1, and pparγ and significantly increased the expression of cpt1. Results of the present study indicated that moderate tributyrin supplementation in diet containing high level of soybean oil could promote growth performance and reduce liver fat accumulation and hyperlipidemia of cultured juvenile large yellow croaker, which could be due to the decrease in lipogenesis‐related genes expression and the increase in lipid oxidation‐related genes expression.

中文翻译：

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日粮三丁酸甘油酯对高豆油饲喂的少年大黄鱼（Larimichthys crocea）的生长性能，身体成分，血清生化指标和脂质代谢相关基因表达的影响

进行了为期10周的饲料试验，以研究补充三丁精（TB）对少年大黄鱼（Larimichthys crocea）的生长性能，身体成分，血清生化指标和脂质代谢相关基因表达的影响）喂食含高水平豆油的饮食。在阴性对照组中，80 g / kg鱼油被用作唯一的脂质来源（FO组），而在阳性对照组中，20 g / kg鱼油和60 g / kg大豆油被用作脂质来源。 （FS组）。在阳性对照饮食的基础上，分别添加1、2、4和8 g / kg的三丁酸甘油酯。结果表明，与阴性对照组相比，高膳食大豆油显着降低了幼年大黄鱼的生长性能，并增加了其肝脏脂质的沉积。与阳性对照组相比，补充2 g / kg的三丁酸甘油酯显着提高了幼年大黄鱼的生长性能，并降低了其肝脏甘油三酸酯和血清胆固醇的含量。补充4 g / kg的三丁酸甘油酯可显着降低幼年大黄鱼的肝脏甘油三酸酯和血清胆固醇及游离脂肪酸含量。此外，补充2和4 g / kg的三丁酸甘油酯显着降低了acc，srebp-1和pparγ并显着增加cpt1的表达。本研究结果表明，在豆油含量高的饮食中适量添加三丁酸甘油酯可以促进生长的性能，并减少养殖的大黄鱼的肝脏脂肪积累和高脂血症，这可能是由于与脂肪形成相关的基因表达和脂肪含量下降所致。脂质氧化相关基因表达的增加。