Propolis is a naturally produced resinous mixture the bees are secreted through collecting resin of plants, buds, and exudates. According to several studies, propolis possessed various properties, one of which known as immunomodulatory property. Immunomodulatory property can be assessed and observed through the anti-inflammatory and cytotoxicity properties of propolis. In Indonesia, Tetragonula sapiens (T. sapiens) from Sulawesi is considered as one of the most commercial and productive bees. Despite of their productivity and commerciality, they have never been tested for their immunomodulatory property. In order to explore their potential even further, this present study was aimed to observe the anti-inflammatory and cytotoxicity properties of T. sapiens propolis from Sulawesi, Indonesia against RAW 264.7 cell lines. The cytotoxic assay of propolis against RAW264.7 cell lines was conducted using MTS assay. The anti-inflammatory properties were determined using LPS-induced RAW 264.7 macrophages cells to measure inhibitory activity of propolis on production of tumor necrosis factor-alpha (TNF-α), inducible nitric oxide synthase (iNOS), and nitric oxide (NO). The cytotoxic test results showed that propolis extract concentration affected the cell in terms of increasing or decreasing the cell viability. The results of the anti-inflammatory test disclosed that propolis at 120 µg/mL had an anti-inflammatory property which was evidenced by TNF-α levels of 304.28 ± 30.25 pg/mL, iNOS of 5.42 ± 0.82 ng/mL, and NO 101.09 ± 1.49 µmol/L. It can be concluded that the Indonesian propolis from T. sapiens possess anti-inflammatory properties related to the inhibition of NO production by macrophages.

蜂胶是一种天然产生的树脂混合物，蜜蜂是通过收集植物，芽和渗出液的树脂来分泌的。根据几项研究，蜂胶具有多种特性，其中之一被称为免疫调节特性。可以通过蜂胶的抗炎和细胞毒性特性来评估和观察其免疫调节特性。在印度尼西亚，来自苏拉威西岛的四角锥（T. sapiens）被认为是最商业和最具生产力的蜜蜂之一。尽管它们具有生产力和商业性，但从未对其免疫调节特性进行过测试。为了进一步探索其潜力，本研究旨在观察智人的抗炎和细胞毒性特性。来自印度尼西亚苏拉威西岛的蜂胶对抗RAW 264.7细胞系。蜂胶对RAW264.7细胞系的细胞毒性测定采用MTS测定进行。使用LPS诱导的RAW 264.7巨噬细胞测定其抗炎特性，以测量蜂胶对肿瘤坏死因子-α（TNF-α），诱导型一氧化氮合酶（iNOS）和一氧化氮（NO）产生的抑制活性。细胞毒性测试结果表明，蜂胶提取物的浓度影响细胞活力的增加或降低。抗炎测试结果表明，蜂胶含量为120 µg / mL时具有抗炎特性，TNF-α含量为304.28±30.25 pg / mL，iNOS为5.42±0.82 ng / mL，NO为101.09证明了这一点。 ±1.49 µmol / L。可以得出结论，印尼蜂胶来自智木具有抗炎特性，与巨噬细胞抑制NO产生有关。