Droplet-based single-cell RNA sequence analyses assume that all acquired RNAs are endogenous to cells. However, any cell-free RNAs contained within the input solution are also captured by these assays. This sequencing of cell-free RNA constitutes a background contamination that confounds the biological interpretation of single-cell transcriptomic data.

中文翻译：

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SoupX 从基于液滴的单细胞 RNA 测序数据中去除环境 RNA 污染


基于液滴的单细胞 RNA 序列分析假设所有获得的 RNA 都是细胞内源性的。然而，这些检测也会捕获输入溶液中包含的任何无细胞 RNA。这种无细胞 RNA 测序构成了背景污染，混淆了单细胞转录组数据的生物学解释。