ABSTRACT

The preparation of SEM samples is a complex and time-consuming process. The present report describes the development of a simple and inexpensive high pressure water freeze fracture method for the preparation of SEM samples from hydrogels without using organic solvents. This method involves two key features. First, the sample and water are frozen with liquid nitrogen in a pressure-resistant container, and the sample is pressurized by volume expansion due to phase transition of the water around the sample as it freezes, which suppresses the growth of ice crystals due to the increase in viscosity of the water inside and around the sample. Second, a cross-section of the sample is prepared without the use of a knife. This method is expected to contribute significantly to biological and food science research.

抽象的

SEM样品的制备是一个复杂且耗时的过程。本报告描述了一种简单而廉价的高压水冻裂方法的开发，该方法无需使用有机溶剂即可从水凝胶制备SEM样品。此方法涉及两个关键功能。首先，将样品和水用液氮冷冻在一个耐压容器中，并且由于样品冻结时水在样品周围的相变而通过体积膨胀对样品加压，从而抑制了冰晶的生长。增加样品内部和周围的水的粘度。其次，不使用刀就制备样品的横截面。预计该方法将对生物学和食品科学研究做出重大贡献。