Plant WRKY transcriptional factors play an important role in responses to biotic and abiotic stresses and are involved in a wide range of resistance strategies against pathogen attacks. The present study investigated the functional role of FaWRKY11 in the regulation of defense responses to B. cinerea in strawberry fruit (Fragaria ananassa Duch. cv. Benihoppe). As strawberry fruits ripened and developed, FaWRKY11 expression levels gradually decreased and were closely related to the severity of B. cinerea. Transient transformation was conducted to overexpress and silence FaWRKY11 in strawberry fruits, which were later analyzed upon B. cinerea inoculation. The results indicated that 5 days following infestation with B. cinerea, the tissue damage of the fruits with overexpressed FaWRKY11 was significantly alleviated. The disease incidence was significantly lower that of the control. Conversely, the degree of damage suffered by the silenced fruit tissue increased to a certain extent, and the disease incidence was slightly higher than that of the control. The results revealed the positive regulatory function of FaWRKY11 in the resistance of strawberries against B. cinerea. Once the FaWRKY11 gene was overexpressed and silenced, the expression levels of the genes related to B. cinerea of the fruit were regulated to diverse extents. The most significant variations were observed in the expression levels of transcription factors related to disease resistance, and also in genes related to metabolism of phytohormones. Our study inferred that the FaWRKY11 might regulate the resistance of strawberries against B. cinerea through regulating some disease-resistance transcription factors and metabolic genes. Our study demonstrated the regulatory mechanism of FaWRKY11 regarding the defense in strawberries against B. cinerea.

植物WRKY转录因子在对生物和非生物胁迫的响应中起着重要作用，并且参与了多种针对病原体攻击的抗性策略。本研究调查了FaWRKY11在调节草莓果实中对灰葡萄孢的防御反应中的功能性作用（Fragaria ananassa Duch。cv。Benihoppe）。随着草莓果实的成熟和发育，FaWRKY11表达水平逐渐降低，并且与灰质芽孢杆菌的严重程度密切相关。进行了瞬时转化，以过度表达和沉默草莓果实中的FaWRKY11，随后对其进行了灰葡萄孢分析。接种。结果表明，用灰质芽孢杆菌侵染5天后，过量表达FaWRKY11的果实的组织损伤得到明显减轻。该疾病的发生率明显低于对照组。相反，沉默的果实组织遭受的损害程度在一定程度上增加，并且该疾病的发病率略高于对照组。结果表明FaWRKY11在草莓对灰霉病的抗性中具有积极的调控作用。一旦FaWRKY11基因过表达和沉默，所述基因的表达水平相关的灰霉病对水果的调节程度不同。在与疾病抗性有关的转录因子的表达水平以及与植物激素代谢有关的基因中观察到最显着的变化。我们的研究推断，FaWRKY11可能通过调节某些抗病转录因子和代谢基因来调节草莓对灰葡萄孢的抗性。我们的研究证明了FaWRKY11调控草莓对灰霉病的防御作用。