OBJECTIVE The rapid accumulation of crude-oil based plastics in the environment is posing a fundamental threat to the future of mankind. The biodegradable and bio-based polyhydroxyalkanoates (PHAs) can replace conventional plastics, however, their current production costs are not competitive and therefore prohibiting PHAs from fulfilling their potential. RESULTS Different low-quality animal by-products, which were separated by thermal hydrolysis into a fat-, fat/protein-emulsion- and mineral-fat-mixture- (material with high ash content) phase, were successfully screened as carbon sources for the production of PHA. Thereby, Ralstonia eutropha Re2058/pCB113 accumulated the short- and medium-chain-length copolymer poly(hydroxybutyrate-co-hydroxyhexanoate) [P(HB-co-HHx)]. Up to 90 wt% PHA per cell dry weight with HHx-contents of 12-26 mol% were produced in shake flask cultivations. CONCLUSION In future, the PHA production cost could be lowered by using the described animal by-product streams as feedstock.

中文翻译：

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用于生产 PHA 生物聚合物的低质量动物副产品流：脂肪、脂肪/蛋白质乳液和高灰分含量的材料作为低成本原料

目的 原油基塑料在环境中的快速积累对人类的未来构成根本威胁。可生物降解的生物基聚羟基脂肪酸酯 (PHA) 可以取代传统塑料，但其目前的生产成本不具有竞争力，因此阻碍了 PHA 发挥其潜力。结果通过热水解将不同的低质量动物副产品分离成脂肪相、脂肪/蛋白质乳液相和矿物脂肪混合物相（灰分含量高的材料）相，成功筛选出作为碳源的碳源。 PHA的生产。由此，富养产碱菌Re2058/pCB113积累了短链和中链长度的共聚物聚(羟基丁酸酯-共-羟基己酸酯)[P(HB-co-HHx)]。在摇瓶培养中，每细胞干重可产生高达 90 wt% 的 PHA，HHx 含量为 12-26 mol%。结论 将来，通过使用所描述的动物副产品流作为原料可以降低 PHA 的生产成本。