CXCL6 fuels the growth and metastases of esophageal squamous cell carcinoma cells both in vitro and in vivo through upregulation of PD‐L1 via activation of STAT3 pathway,Journal of Cellular Physiology

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CXCL6 fuels the growth and metastases of esophageal squamous cell carcinoma cells both in vitro and in vivo through upregulation of PD‐L1 via activation of STAT3 pathway
Journal of Cellular Physiology ( IF 4.5 ) Pub Date : 2020-12-23 , DOI: 10.1002/jcp.30236
Shutao Zheng ₁ , Tongxue Shen ₁ , Qing Liu ₁ , Tao Liu ₂ , Aerziguli Tuerxun ₁ , Qiqi Zhang ₁ , Lifei Yang ₃ , Xiujuan Han ₁ , Xiaomei Lu _{1,

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Affiliation

CXCL6, contraction of C‐X‐C motif chemokine ligand 6, whose biological roles have been rarely described in esophageal squamous cell carcinoma (ESCC). To understand the clinicopathological and biological roles played by CXCL6 in the growth and metastasis of ESCC, immunohistochemistry was used to detect the expression of CXCL6 in ESCC tissues, totaling 105 cases; and the correlation was statistically analyzed between CXCL6 expression and clinicopathological parameters. The role mediated in migration and invasion was evaluated using wound‐healing and Transwell assays. MTT and flow cytometry were used to assay the proliferative variation. In vivo, tail vein injection model was established in nude mice xenografted with human ESCC cell lines whose CXCL6 were artificially manipulated. It was found that relative to normal control, CXCL6 was profoundly higher in ESCC; upregulated CXCL6 only significantly correlated with differentiation degree. In vitro, CXCL6 was found to promote the proliferation, migration, and invasion of ESCC cells; which was fully corroborated by nude mice experiment that CXCL6 can promote the growth and metastases of ESCC cells in vivo. Mechanistically, CXCL6 was discovered to be capable of promoting epithelial‐mesenchymal transition and upregulating PD‐L1 expression through activation of the STAT3 pathway. Collectively, all the data we showed here demonstrate that CXCL6 can enhance the growth and metastases of ESCC cells both in vivo and in vitro.

中文翻译：

CXCL6通过激活STAT3通路上调PD-L1促进体外和体内食管鳞状细胞癌细胞的生长和转移

CXCL6，C-X-C 基序趋化因子配体 6 的收缩，其生物学作用在食管鳞状细胞癌 (ESCC) 中很少被描述。为了解CXCL6在ESCC生长和转移中的临床病理和生物学作用，采用免疫组化检测CXCL6在ESCC组织中的表达，共105例；并对CXCL6表达与临床病理参数的相关性进行统计分析。使用伤口愈合和 Transwell 试验评估在迁移和侵袭中介导的作用。MTT和流式细胞术用于测定增殖变异。在体内，在裸鼠中建立了尾静脉注射模型，该模型是用人工操纵的CXCL6的人ESCC细胞系异种移植的。发现相对于正常对照，CXCL6 在 ESCC 中明显更高；CXCL6 上调仅与分化程度显着相关。在体外，发现CXCL6促进ESCC细胞的增殖、迁移和侵袭；裸鼠实验充分证实了CXCL6可以促进体内ESCC细胞的生长和转移。从机制上讲，CXCL6 被发现能够通过激活 STAT3 通路促进上皮间质转化和上调 PD-L1 表达。总的来说，我们在这里展示的所有数据都表明 CXCL6 可以在体内和体外促进 ESCC 细胞的生长和转移。裸鼠实验充分证实了CXCL6可以促进体内ESCC细胞的生长和转移。在机制上，发现 CXCL6 能够通过激活 STAT3 通路促进上皮间质转化和上调 PD-L1 表达。总的来说，我们在这里展示的所有数据都表明 CXCL6 可以在体内和体外促进 ESCC 细胞的生长和转移。裸鼠实验充分证实了CXCL6可以促进体内ESCC细胞的生长和转移。在机制上，发现 CXCL6 能够通过激活 STAT3 通路促进上皮间质转化和上调 PD-L1 表达。总的来说，我们在这里展示的所有数据都表明 CXCL6 可以在体内和体外促进 ESCC 细胞的生长和转移。

更新日期：2020-12-23

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