Centaurea depressa, a medicinal herb used in traditional Ladakhi medicine is classified as vulnerable under the IUCN Red data book. Reports on its in vitro micropropagation for conservation of this species is however lacking. This study showed that nodal explants multiplied (11.75 shoot buds/node) when BA (2.22 µM) was used while in KN lesser number of multiple shoots were induced. NAA even at the lowest concentrations (0.54µM) completely reduced this response and induced mostly calli. The most interesting result was the induction of shoots (5.2± 0.39 shoot buds induced/explant) from root/rhizomatous explants of this species incubated in light (16 h) and cultured in ½ MS basal medium with BA (4.44 µM). Anatomy of the regenerated vascular traces in the induced shoot confirmed direct organogenesis and is a useful method to propagate this endangered species. DNA phylogenetic analysis with universal barcoding primers (ITS1, rbcL, matK, psbA-trnfH and ycf1) using total DNA isolated from the tissue cultured propagules is presented.

矢车菊IUCN Red数据手册将传统Ladakhi药物中使用的草药列为易受伤害的物种。但是，缺乏有关其在体外微繁殖中保护该物种的报道。这项研究表明，当使用BA（2.22 µM）时，结节外植体成倍增加（11.75个芽/结），而在KN中，诱导的多芽数量较少。NAA甚至在最低浓度（0.54µM）时也完全降低了这种反应，并主要诱导了愈伤组织。最有趣的结果是从该物种的根/根瘤性外植体在光下孵育（16 h）诱导出芽（诱导/移出5.2±0.39个芽），并在含BA（4.44 µM）的1/2 MS基础培养基中培养。诱导枝条中再生血管痕迹的解剖证实了直接的器官发生，并且是繁殖这种濒危物种的有用方法。