The differential expression of many productive and reproductive traits has already been associated with the genotype effect observed between Bos taurus indicus (Bos indicus) and Bos taurus taurus (Bos taurus) breeds. Lipids have a crucial role during oocyte development. The aim of this work was to characterize the lipid profiles of oocytes recovered from dairy breeds: Gir (Bos indicus), Holstein (Bos taurus) and their crossbred (Gir x Holstein). Cumulus-oocyte complexes were collected by ovum pick-up from Gir (n=21), Holstein (n=36), and crossbreed (n=33) cows and submitted to MALDI-MS lipid fingerprint analysis. Multi- and univariate analyses were used to characterize lipid profiles of oocytes derived from each breed. Third lipid profiles (Gir n=7; Holstein n=12; and crossbred n=11) were evaluated and allowed characterization of oocyte's lipids among breeds. The lipid profiles from Gir and Holstein were observed to be the most distinct, whereas Gir and crossbred oocytes presented greater similarities. Overall, a wide-range oocyte lipid profile characterization from distinctive bovine dairy breeds recovered by OPU were provided. We have identified two distinguishable lipid profile patterns among groups and revealed that phospholipids and triacylglycerol were the predominantly lipid assigned in Gir (Bos indicus) and Holstein (Bos taurus) oocytes, respectively.

许多生产性状和生殖性状的差异表达已经与印度双头金牛（Bos indicus）和非洲双头金牛（Bos taurus）品种的基因型效应有关。脂质在卵母细胞发育过程中起着至关重要的作用。这项工作的目的是表征从乳牛品种中回收的卵母细胞的脂质谱：Gir（Bos indicus），Holstein（Bos taurus）和它们的杂交种（Gir x Holstein）。通过卵子采集从吉尔（n = 21），荷斯坦（n = 36）和杂种（n = 33）母牛收集卵-卵母细胞复合物，并进行MALDI-MS脂质指纹分析。使用多变量和单变量分析来表征每个品种衍生的卵母细胞的脂质谱。评估了第三类脂质谱（Gir n = 7； Holstein n = 12；杂种n = 11），并表征了不同品种的卵母细胞脂质。观察到来自Gir和Holstein的脂质谱最明显，而Gir和杂交卵母细胞的相似性更高。总体而言，提供了由OPU回收的独特牛乳种的大范围卵母细胞脂质谱特征。Bos indicus和Holstein（Bos taurus）卵母细胞。