Abstract

A new neurotoxin RTX-VI that modulates the voltage-gated sodium channels (Na_V) was isolated from the ethanolic extract of the sea anemone Heteractis crispa. Its amino acid sequence was determined using the combination of Edman degradation and tandem mass spectrometry. RTX-VI turned out to be an unusual natural analogue of the previously described sea anemone toxin RTX-III. The RTX-VI molecule consists of two disulfide-linked peptide chains and is devoid of Arg13, which is important for the selectivity and affinity of such peptides for the Na_V channels. Electrophysiological screening of RTV-VI on Na_V channel subtypes showed its selective interaction with the central nervous system (Na_V1.2, Na_V1.6) and insect (BgNa_V1, VdNa_V1) sodium channels.

中文翻译：

---

新型海葵毒素RTX-VI选择性调节电压门控钠通道

摘要

从海葵Heteractis crispa的乙醇提取物中分离出一种新的神经毒素RTX-VI，它调节电压门控钠通道（Na _V）。使用Edman降解法和串联质谱法确定其氨基酸序列。事实证明，RTX-VI是先前描述的海葵毒素RTX-III的不寻常天然类似物。RTX-VI分子由两条二硫键连接的肽链组成，不含Arg13，这对于此类肽对Na _V通道的选择性和亲和力很重要。RTV-VI对Na _V通道亚型的电生理检查显示其与中枢神经系统的选择性相互作用（Na _V 1.2，Na _V1.6）和昆虫（BgNa _V 1，VdNa _V 1）钠通道。