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New Sea Anemone Toxin RTX-VI Selectively Modulates Voltage-Gated Sodium Channels
Doklady Biochemistry and Biophysics ( IF 0.8 ) Pub Date : 2020-12-25 , DOI: 10.1134/s1607672920060071
R S Kalina 1 , S Peigneur 2 , I N Gladkikh 1 , P S Dmitrenok 1 , N Y Kim 1 , E V Leychenko 1 , M M Monastyrnaya 1 , J Tytgat 2 , E P Kozlovskaya 1
Affiliation  

Abstract

A new neurotoxin RTX-VI that modulates the voltage-gated sodium channels (NaV) was isolated from the ethanolic extract of the sea anemone Heteractis crispa. Its amino acid sequence was determined using the combination of Edman degradation and tandem mass spectrometry. RTX-VI turned out to be an unusual natural analogue of the previously described sea anemone toxin RTX-III. The RTX-VI molecule consists of two disulfide-linked peptide chains and is devoid of Arg13, which is important for the selectivity and affinity of such peptides for the NaV channels. Electrophysiological screening of RTV-VI on NaV channel subtypes showed its selective interaction with the central nervous system (NaV1.2, NaV1.6) and insect (BgNaV1, VdNaV1) sodium channels.



中文翻译:

新型海葵毒素RTX-VI选择性调节电压门控钠通道

摘要

从海葵Heteractis crispa的乙醇提取物中分离出一种新的神经毒素RTX-VI,它调节电压门控钠通道(Na V)。使用Edman降解法和串联质谱法确定其氨基酸序列。事实证明,RTX-VI是先前描述的海葵毒素RTX-III的不寻常天然类似物。RTX-VI分子由两条二硫键连接的肽链组成,不含Arg13,这对于此类肽对Na V通道的选择性和亲和力很重要。RTV-VI对Na V通道亚型的电生理检查显示其与中枢神经系统的选择性相互作用(Na V 1.2,Na V1.6)和昆虫(BgNa V 1,VdNa V 1)钠通道。

更新日期:2020-12-25
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