Abstract

Genes homologous to PSY1 and PSY2 that encode phytoene synthase isoforms in Capsicum species C. baccatum, C. chinense, C. frutescens, C. tovarii, C. eximium, and C. chacoense were identified. High conservatism of functionally significant sites of phytoene synthases of the analyzed accessions was revealed. It was found that only PSY1-based clustering of pepper species corresponds to the traditional Capsicum phylogeny; C. eximium was a part of the Purple corolla complex, and C. chacoense was equidistant from Annuum and Baccatum clades. The absence of significant differences between PSY1 and PSY2 of yellow-fruited C. chinense and red-fruited pepper accessions was shown. The yellow color of C. chinense fruit may be the result of both decreased PSY1 expression and increased PSY2 transcription. Thus, it was demonstrated that the acquired fruit pigmentation retains strict phylogenetic limitations, which, however, can be overcome using artificial selection for the activity of phytoene synthase PSY1.

中文翻译：

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不同辣椒复合物种类中辣椒植物苯合酶旁系物PSY1和PSY2的变异性和系统发育。

摘要

基因同源PSY1和PSY2在八氢番茄红素编码合酶亚型辣椒物种风铃，C.楸，C.紫苏，C. tovarii，C. eximium，和C. chacoense进行鉴定。揭示了所分析种质的八氢番茄红素合酶的功能重要位点的高度保守性。发现只有基于PSY1的辣椒种簇才对应于传统的辣椒系统发育。C. eximium是紫色花冠复合体的一部分，而C. chacoense与Annuum和Baccatum进化枝等距。结果表明，黄色果实的C. chinense和红色果实的胡椒品种的PSY1和PSY2之间没有显着差异。C. chinense果实的黄色可能是PSY1表达减少和PSY2转录增加的结果。因此，证明了获得的水果色素沉着保留了严格的系统发育限制，但是，可以通过使用人工选择来获得植物六烯合酶PSY1的活性。