Bioconjugation of 2-arachidonoyl glycerol (2-AG) biotinylated antibody with gold nano-flowers toward immunosensing of 2-AG in human plasma samples: A novel immuno-platform for the screening of immunomodulation and neuroprotection using biosensing,Analytical Methods

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Bioconjugation of 2-arachidonoyl glycerol (2-AG) biotinylated antibody with gold nano-flowers toward immunosensing of 2-AG in human plasma samples: A novel immuno-platform for the screening of immunomodulation and neuroprotection using biosensing
Analytical Methods ( IF 2.7 ) Pub Date : 2020-12-8 , DOI: 10.1039/d0ay02135k
Ahmad Mobed ₁ , Fereshteh Kohansal , Ali Ahmadalipour , Mohammad Hasanzadeh , Flor Zargari

Affiliation

Human 2-arachidonoylglycerol (2-AG) is an agonist of endocannabinoid system and acts as an important modulator of many physiological processes such as emotional state and pain sensation. Identification and quantification of 2-AG is vital for medical and pathological processes. There are no reports on the measurement of 2-AG in human biofluids using modern methods such as biosensors. This study reports an ultra-sensitive and selective immunosensor to determine endocannabinoids 2-AG in human plasma samples. In this study, gold nano-flowers (AuNFs) were synthesized and conjugated with a specific biotinylated antibody of 2-AG. Bioconjugated composite (bioreceptor with AuNFs) was immobilized on the surface of a gold electrode and used for the monitoring of the antigen (target molecules) based on the immunoreaction process. Moreover, a constructed interface was characterized by field-emission scanning electron microscopy (FE-SEM), dynamic light scattering (DLS), transmission electron microscopy (TEM) and zeta potential methods. Using the proposed immuno-platform, 2-AG was determined in two dynamic ranges of 0.00024–0.0078 ng L⁻¹ and 2–16 ng L⁻¹ with a lower limit of quantitation (LLOQ) of 0.00024 ng L⁻¹. These results suggest that our immunosensor might be appropriate for an early diagnosis of 2-AG towards the screening of immunomodulatory activity and neuroprotection.

中文翻译：

2-花生四烯酸甘油 (2-AG) 生物素化抗体与金纳米花对人血浆样品中 2-AG 的免疫传感的生物偶联：一种使用生物传感筛选免疫调节和神经保护的新型免疫平台

人 2-花生四烯酸甘油 (2-AG) 是内源性大麻素系统的激动剂，是情绪状态和疼痛感等许多生理过程的重要调节剂。2-AG 的鉴定和定量对于医学和病理过程至关重要。没有关于使用生物传感器等现代方法测量人体生物体液中 2-AG 的报道。这项研究报告了一种超灵敏和选择性的免疫传感器，用于测定人血浆样品中的内源性大麻素 2-AG。在这项研究中，合成了金纳米花 (AuNF)，并与 2-AG 的特异性生物素化抗体偶联。将生物共轭复合物（具有 AuNFs 的生物受体）固定在金电极表面，用于基于免疫反应过程监测抗原（靶分子）。而且，通过场发射扫描电子显微镜（FE-SEM）、动态光散射（DLS）、透射电子显微镜（TEM）和zeta电位方法对构建的界面进行了表征。使用建议的免疫平台，在 0.00024–0.0078 ng L 的两个动态范围内测定 2-AG^-1和 2–16 ng L^-1的定量下限 (LLOQ) 为 0.00024 ng L^-1。这些结果表明，我们的免疫传感器可能适用于 2-AG 的早期诊断，以筛查免疫调节活性和神经保护。

更新日期：2020-12-24

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