The regeneration of bone and tooth tissues, and related cellular therapies, has attracted widespread attention. Bone marrow mesenchymal stem cells (BMSCs) are potential candidates for such regeneration. iRoot SP is a premixed bioceramic root canal sealer widely used in clinical settings. However, the effect of iRoot SP on the biological features of BMSCs has not been elucidated. In the present study, we found that 0.2 mg/ml iRoot SP conditioned medium promoted osteo/odontogenic differentiation and enhanced mineralization of BMSCs without affecting the proliferative ability. Mechanistically, the NF-κB and MAPK signaling pathways were activated in SP-treated BMSCs, and differentiation was inhibited when cultured with the specific inhibitor. Taken together, these findings demonstrate that iRoot SP promotes osteo/odontogenic differentiation of BMSCs via the NF-κB and MAPK signaling pathways, which could provide a new theoretical basis for clinical applications of iRoot SP and a new therapeutic target for the regeneration of bone and tooth tissue in the future.

中文翻译：

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iRoo​​t SP通过激活NF-κB和MAPK信号通路促进骨髓间充质干细胞的成骨/成牙作用

骨骼和牙齿组织的再生以及相关的细胞疗法引起了广泛的关注。骨髓间充质干细胞（BMSC）是这种再生的潜在候选人。iRoo​​t SP是一种预混合的生物陶瓷根管封闭剂，广泛用于临床环境。但是，尚未阐明iRoot SP对BMSC生物学特性的影响。在本研究中，我们发现0.2 mg / ml iRoot SP条件培养基可促进BMSCs的成骨/牙源性分化和矿化，而不会影响其增殖能力。从机制上讲，NF- κB和MAPK信号通路在SP处理的BMSC中被激活，并且在与特异性抑制剂一起培养时可抑制分化。总之，这些发现证明，iRoot SP促进骨/经由NF-骨髓干细胞的牙源性分化κ B和MAPK信号传导途径，其可以用于骨的再生iRoo​​t SP和治疗的新靶点的临床应用提供了新的理论依据和将来的牙齿组织。