Abstract

A novel coordination polymer (CP) {[Eu(HL)]·2(H₂O)}_n (1) contains luminescent Eu^III ion as the metal node on the basis of a bis-(imidazole-4,5-dicarboxylate) ligand (H₄L) was synthesized via reaction of H₄L and Eu(NO₃)₃·6H₂O using sodium hydroxide as pH regulator under the hydrothermal condition. The inhibitory activity of compound on SW16 (human colon cancer cells) with squamous shape was further evaluated. Reverse transcription polymerase chain reaction (RT-PCR) was used to assess Tribbles homolog 3 (trib3) gene relative expression level after compound treatment. Besides, Cell Counting Kit-8 (CCK-8) was used to detect the proliferation of SW16 colon cancer cells, and transwell was performed to detect the migration and invasion ability of SW16 cells. Ultimately, the results of molecular docking simulation reveal the interaction of Tribbles homolog 3 (TRIB3) protein and Eu^III metallic complex.

摘要

一种新型配位聚合物 (CP) {[Eu(HL)]·2(H ₂ O)} _n ( 1 ) 包含发光的 Eu ^III离子作为基于双-(咪唑-4,5-二羧酸盐)的金属节点)配体(H ₄ L)是通过H ₄ L和Eu(NO ₃ ) ₃ ·6H ₂ O在水热条件下使用氢氧化钠作为pH调节剂的反应合成的。进一步评估了化合物对具有鳞状形状的SW16（人结肠癌细胞）的抑制活性。逆转录聚合酶链反应 (RT-PCR) 用于评估 Tribbles 同源物 3 ( trib3) 化合物处理后的基因相对表达水平。此外，使用Cell Counting Kit-8（CCK-8）检测SW16结肠癌细胞的增殖情况，并通过transwell检测SW16细胞的迁移和侵袭能力。最终，分子对接模拟的结果揭示了 Tribbles 同源物 3 (TRIB3) 蛋白与 Eu ^III金属络合物的相互作用。