ABSTRACT

In this study, we aimed to synthesize, characterize, and investigate the efficacy of silver nanoparticles (AgNPs) derived from the fruit and leaf extracts of C. ambigua against free radical, bacterial strains and malarial parasites. Their antibacterial, antimalarial and antioxidant efficacy were examined by micro-dilution, parasite viability, and spectrophotometric techniques respectively. The result of characterization of the silver nanoparticles showed that biosynthesis of AgNPs was successful. FTIR analysis showed peak around 500 cm⁻¹ ascribed to Ag-O peak for both nanoparticles. Also, spherically shaped materials from TEM images indicated that AgNPs were obtained. The AgNPs exhibited strong inhibitory activity on the seven test bacterial strains with minimum inhibitory concentration (MIC) ranging between 3.25 –30.00 mg mL⁻¹ and 15.00 – 30.00 mg mL⁻¹ on the Gram-positive and Gram-negative bacterial strains respectively. The efficacy of AgNPs to reduce 2, 2-diphenyl-1-picrylhydrazy (DPPH) radical to non-radical molecule (DPPH-H) was significant and the IC₅₀ of AgNPs mediated by leaf extract was 0.21 and 0.80 mg mL⁻¹ for the fruit silver nanoparticles. Similarly, the synthesized nanoparticles from the fruit and leaf parts of the plant showed excellent antimalarial properties with % inhibition of 100 at 20 μg mL⁻¹ compared to the plant extract with lower % inhibition (48.5%). The AgNPs were observed to inhibit bacterial strains and have a very strong antioxidant and antimalarial activities indicating that plant parts of C. ambigua are very good precursors for non-artificial anti-microbial, antioxidant and antimalarial drugs.

摘要

在这项研究中，我们旨在合成，表征和研究源自C. ambigua的果实和叶片提取物的银纳米颗粒（AgNP）对自由基，细菌菌株和疟疾寄生虫的功效。分别通过微量稀释，寄生虫生存力和分光光度法检查了它们的抗菌，抗疟和抗氧化功效。银纳米颗粒的表征结果表明，AgNPs的生物合成是成功的。FTIR分析显示峰在500 cm ^-1附近归因于两个纳米粒子的Ag-O峰。另外，由TEM图像得到的球形材料表明获得了AgNP。所述的AgNPs上以最小抑制浓度（MIC）3.25 -30.00毫克毫升之间的范围内的七个试验的细菌菌株显示出强抑制活性^-1 30.00毫克毫升-和15.00 ^-1分别对革兰氏阳性和革兰氏阴性细菌菌株。AgNPs将2，2-二苯基-1-吡啶并羟基（DPPH）自由基还原为非自由基分子（DPPH-H）的功效非常显着，并且叶提取物介导的AgNPs的IC ₅₀为0.21和0.80 mg mL ^-1用于水果银纳米颗粒。类似地，与植物提取物相比，植物的果实和叶部分合成的纳米颗粒显示出优异的抗疟特性，在20μgmL ^-1下的抑制百分比为100，而抑制率较低的植物提取物（48.5％）。观察到AgNP抑制细菌菌株并且具有非常强的抗氧化和抗疟活性，这表明C. ambigua的植物部分是非人工抗微生物，抗氧化和抗疟药物的非常好的前体。