ABSTRACT

Background: The aim of this study was to measure the level of translocator protein (TSPO) in patients with intracerebral hematoma (ICH) and to determine whether TSPO can predict ICH outcomes.

Method: Patients with ICH were recruited at Wujin Hospital Affiliated with Jiangsu University between January 2018 and May 2020. The level of TSPO and inflammatory factors were analyzed by enzyme-linked immunosorbent assay (ELISA). A receiver operating characteristic curve (ROC) analysis was applied to assess the accuracy of TSPO for predicting patient outcomes.

Result: The median of TSPO was 2.26 ng/ml. The lower- (46 cases) and higher-(51 cases) TSPO groups were thus divided based on the median value. The perihematomal edema (PHE) volume in the lower TSPO group was 6.3 ± 1.3 ml which was significantly lower than that in higher-TSPO group (14.8 ± 3.5 ml) (p < 0.05). The serum level of the interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and C-reactive protein (CRP) in the higher-TSPO group was significantly higher than that in the lower TSPO group (p < 0.05). The Spearman’s correlation found that TSPO concentrations significantly correlated with PHE volume, modified Rankin Scale score (MRS), IL-1β, IL-6, TNF-α, and CRP concentrations. The area under the ROC (AUC), specificity, sensitivity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), and Diagnostic Odds Ratio (DOR) of TSPO was 0.932, 82.1%, 89.9%, 5.02, 0.12, and 40.8, respectively, which was more reliable than other inflammatory factors.

Conclusion: The TSPO may a reliable biomarker in predicting the prognosis of ICH patients.

摘要

背景：本研究的目的是测量脑内血肿 (ICH) 患者的易位蛋白 (TSPO) 水平，并确定 TSPO 是否可以预测 ICH 结果。

方法：2018年1月至2020年5月在江苏大学附属武进医院招募ICH患者，采用酶联免疫吸附试验（ELISA）分析TSPO和炎症因子水平。应用受试者工作特征曲线 (ROC) 分析来评估 TSPO 预测患者结果的准确性。

结果：TSPO 的中位数为 2.26 ng/ml。因此基于中值划分较低（46 例）和较高（51 例）TSPO 组。低 TSPO 组的血肿周围水肿 (PHE) 体积为 6.3 ± 1.3 ml，显着低于高 TSPO 组 (14.8 ± 3.5 ml) (p < 0.05)。高TSPO组血清IL-1β（IL-1β）、IL-6（IL-6）、肿瘤坏死因子-α（TNF-α）和C反应蛋白（CRP）水平显着升高高于低 TSPO 组（p < 0.05）。Spearman 相关性发现 TSPO 浓度与 PHE 体积、改良的 Rankin 量表评分 (MRS)、IL-1β、IL-6、TNF-α 和 CRP 浓度显着相关。ROC下面积（AUC）、特异性、敏感性、阳性似然比（PLR）、阴性似然比（NLR）、

结论：TSPO可能是预测ICH患者预后的可靠生物标志物。