Abstract

The objective of this work was to produce and characterise nanoemulsions containing tucumã extract and to evaluate the performance of the nanostructure and the free compound regarding antitumor activity, cytotoxicity, and oxidative metabolism in NB4/APL cells. The nanoemulsions showed adequate physicochemical characteristics (average size approx. 200 nm, polydispersity index less than 0.3, negative zeta potential and acid pH) maintained stable up to 90 days of storage in refrigeration condition. The nanoformulations did not present protein corona formation. Blank nanoemulsion treatments showed moderate toxicity. Furthermore, the nanoemulsion loaded with extract showed better antileukemic results than the free extract. However, nanoemulsions can be promising carriers of natural compounds, emphasising their biological properties and constituting alternatives in treating diseases.

摘要

这项工作的目的是生产和表征含有 tucumã 提取物的纳米乳液，并评估纳米结构和游离化合物在 NB4/APL 细胞中的抗肿瘤活性、细胞毒性和氧化代谢的性能。纳米乳液显示出足够的物理化学特性（平均尺寸约 200 nm，多分散指数小于 0.3，负 zeta 电位和酸性 pH）在冷藏条件下可保持稳定长达 90 天。纳米制剂不存在蛋白质电晕形成。空白纳米乳液处理显示出中等毒性。此外，负载有提取物的纳米乳显示出比游离提取物更好的抗白血病效果。然而，纳米乳液可以成为天然化合物的有前途的载体，