ABSTRACT

Biohythane (hydrogen + methane) production in a two stage dark fermentation (DF) and anaerobic digestion (AD) process from food waste (FW) has been studied. This paper investigated the effect of operation temperature, i.e. mesophilic (34 °C) and thermophilic (55 °C) , on biohythane yield and total energy recovery carried out at the initial culture pH 5.5 and pH 7, respectively for DF and AD batch tests. The mesophilic DF tests gave a higher hydrogen yield of 53.5 ($\pm$4) mL H₂/g VS added compared to thermophilic DF tests, i.e. 37.6 ($\pm$1) mL H₂/g VS added. However, higher methane yields, i.e. 307.5 ($\pm$ 10) mL CH₄/g VS, were obtained at thermophilic AD tests compared to mesophilic AD, i.e. 276.5 ($\pm$4.3) mL CH₄/g VS. The total energy recovery from thermophilic DF + AD was higher (11.4 MJ/kg VS) than the mesophilic (10.4 MJ/kg VS) combined process. Interestingly, the analysis of kinetic parameters of mesophilic tests, determined from the Modified Gompertz equation, showed that mesophilic DF had faster H₂ production kinetics, which can be attributed to a faster adaptation of the heat-shocked inoculum used in the tests to the incubation temperature. However, thermophilic AD tests exhibited faster kinetics for methane production.

摘要

已经研究了在两阶段黑暗发酵 (DF) 和厌氧消化 (AD) 过程中从食物垃圾 (FW) 中生产生物乙烷（氢气 + 甲烷）。本文研究了操作温度的影响，即嗜温（34°C）和嗜热（55°C） ，关于在初始培养物 pH 5.5 和 pH 7 下进行的生物乙烷产量和总能量回收，分别用于 DF 和 AD 批次测试。中温 DF 测试给出了更高的氢产率 53.5 ($\pm$4) 与嗜热 DF 测试相比，添加了 mL H ₂ /g VS，即 37.6 ($\pm$1) 添加mL H ₂ /g VS。然而，更高的甲烷产量，即 307.5 ($\pm$10) mL CH ₄ /g VS，在嗜热 AD 测试中获得，与中温 AD 相比，即 276.5 ($\pm$4.3)mL CH ₄ /g VS。嗜热 DF + AD 的总能量回收率（11.4 MJ/kg VS）高于中温（10.4 MJ/kg VS）联合过程。有趣的是，根据修正的 Gompertz 方程确定的中温测试动力学参数分析表明，中温 DF 具有更快的 H ₂产生动力学，这可归因于测试中使用的热激接种物更快地适应孵化温度。然而，嗜热 AD 测试显示出更快的甲烷产生动力学。