Wastewater treatment plants are considered hot spots for antibiotic resistance. Most studies have addressed the impact on the aquatic environment, as water is an important source of anthropogenic pollutants. Few investigations have been conducted on terrestrial animals living near treatment ponds. We isolated extended-spectrum-β-lactamase Enterobacter cloacae complex-producing strains from 35 clinical isolates, 29 samples of wastewater, 19 wild animals, and 10 domestic animals living in the hospital sewers and at or near a wastewater treatment plant to study the dissemination of clinically relevant resistance through hospital and urban effluents. After comparison of the antibiotic-resistant profiles of E. cloacae complex strains, a more detailed analysis of 41 whole-genome-sequenced strains demonstrated that the most common sequence type, ST114 (n = 20), was present in human (n = 9) and nonhuman (n = 11) samples, with a close genetic relatedness. Whole-genome sequencing confirmed local circulation of this pathogenic lineage in diverse animal species. In addition, nanopore sequencing and specific synteny of an IncHI2/ST1/bla_CTX-M-15 plasmid recovered on the majority of these ST114 clones (n = 18) indicated successful worldwide diffusion of this mobile genetic element.

中文翻译：

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在瓜德罗普岛（法国西部）从医院向附近环境传播产生广谱β-内酰胺酶的阴沟肠杆菌复合物的传播：成功的IncHI2 / ST1质粒的ST114谱系编码

废水处理厂被认为是抗生素耐药性的热点。由于水是人为污染的重要来源，因此大多数研究都针对水环境的影响。对生活在治疗池附近的陆生动物的研究很少。我们从35个临床分离株，29个废水样本，19个野生动物和10个生活在医院下水道和废水处理厂附近的家畜中分离了产生广谱β-内酰胺酶阴沟肠杆菌复合物的菌株，以研究其传播医院和城市污水对临床相关耐药性的影响。比较阴沟肠杆菌的耐药性后复杂的菌株，对41个全基因组测序的菌株进行更详细的分析后发现，人类（n = 9）和非人类（n = 11）样品中存在最常见的序列类型ST114（n = 20），且密切的遗传关联。全基因组测序证实了这种致病谱系在各种动物物种中的局部循环。另外，在大多数这些ST114克隆（n = 18）上回收的IncHI2 / ST1 / bla _CTX-M-15质粒的纳米孔测序和特异同义性表明该移动遗传元件已成功在世界范围内扩散。