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OsmiR164‐targeted OsNAM, a boundary gene, plays important roles in rice leaf and panicle development
The Plant Journal ( IF 6.2 ) Pub Date : 2020-12-23 , DOI: 10.1111/tpj.15143 Zhongyuan Chang 1 , Ruihan Xu 1 , Qian Xun 1 , Jiajun Liu 1 , Tianhui Zhong 1 , Yanfeng Ding 1, 2, 3 , Chengqiang Ding 1, 2, 3
The Plant Journal ( IF 6.2 ) Pub Date : 2020-12-23 , DOI: 10.1111/tpj.15143 Zhongyuan Chang 1 , Ruihan Xu 1 , Qian Xun 1 , Jiajun Liu 1 , Tianhui Zhong 1 , Yanfeng Ding 1, 2, 3 , Chengqiang Ding 1, 2, 3
Affiliation
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The CUP‐SHAPED COTYLEDON (CUC) genes (CUC1, CUC2 and CUC3) regulate organ boundary formation in Arabidopsis. However, the functions of their homologous genes in rice (Oryza sativa) are still unknown. Here, we have identified an orthologous gene of CUC1 and CUC2 in rice, named OsNAM. Subcellular localization and yeast two‐hybrid assay results have suggested that OsNAM encodes a conserved nuclear NAC (NAM/ATAF1/CUC2) protein with a transcriptional activator. The null mutant osnam‐1 presented a fused leaf structure, small panicles, reduced branches and aberrant floral organ identities when compared with those of the wild type. Beta‐glucuronidase staining and GFP reporter lines indicated that OsNAM was expressed in young tissues and that its boundary enrichment expression was regulated by OsmiR164. Loss‐of‐function mutants for OsCUC3 resulted in no obvious defects throughout rice development. The osnam oscuc3 double mutant, however, resulted in severe leaf fusion of the first two leaves, while the osnam single mutant showed a similar phenotype from the seventh leaf. These results indicated that OsNAM and OsCUC3 act redundantly for boundary specification during post‐embryonic development. Overall, we describe the biological functions of OsNAM and OsCUC3 in rice development and the expression characteristics of OsNAM. This work reveals the important role of CUC genes in rice.
中文翻译：
OsmiR164 靶向 OsNAM 是一种边界基因，在水稻叶片和穗发育中起重要作用
所述杯状子叶（CUC）基因（CUC1，CUC2和CUC3）调节器官在拟南芥边界形成。然而，它们的同源基因在水稻（Oryza sativa）中的功能仍然未知。在这里，我们在水稻中鉴定了一个CUC1和CUC2的直系同源基因，命名为OsNAM。亚细胞定位和酵母双杂交试验结果表明OsNAM编码具有转录激活因子的保守核 NAC（NAM/ATAF1/CUC2）蛋白。无效突变体osnam-1与野生型相比，其呈现融合的叶结构、小圆锥花序、减少的分枝和异常的花器官特征。β-葡萄糖醛酸酶染色和 GFP 报告系表明OsNAM在年轻组织中表达，其边界富集表达受OsmiR164调节。OsCUC3的功能丧失突变体在整个水稻发育过程中没有导致明显的缺陷。该osnam oscuc3双突变体，然而，导致了前两个叶严重时叶片融合，而osnam单突变表明从第七叶相似的表型。这些结果表明OsNAM和OsCUC3在胚胎后发育期间对边界规范采取冗余行动。总体而言，我们描述的生物学功能OsNAM和OsCUC3在水稻生长发育和表达特点OsNAM。这项工作揭示了CUC基因在水稻中的重要作用。
更新日期：2020-12-23
中文翻译：
OsmiR164 靶向 OsNAM 是一种边界基因，在水稻叶片和穗发育中起重要作用
所述杯状子叶（CUC）基因（CUC1，CUC2和CUC3）调节器官在拟南芥边界形成。然而，它们的同源基因在水稻（Oryza sativa）中的功能仍然未知。在这里，我们在水稻中鉴定了一个CUC1和CUC2的直系同源基因，命名为OsNAM。亚细胞定位和酵母双杂交试验结果表明OsNAM编码具有转录激活因子的保守核 NAC（NAM/ATAF1/CUC2）蛋白。无效突变体osnam-1与野生型相比，其呈现融合的叶结构、小圆锥花序、减少的分枝和异常的花器官特征。β-葡萄糖醛酸酶染色和 GFP 报告系表明OsNAM在年轻组织中表达，其边界富集表达受OsmiR164调节。OsCUC3的功能丧失突变体在整个水稻发育过程中没有导致明显的缺陷。该osnam oscuc3双突变体，然而，导致了前两个叶严重时叶片融合，而osnam单突变表明从第七叶相似的表型。这些结果表明OsNAM和OsCUC3在胚胎后发育期间对边界规范采取冗余行动。总体而言，我们描述的生物学功能OsNAM和OsCUC3在水稻生长发育和表达特点OsNAM。这项工作揭示了CUC基因在水稻中的重要作用。
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