Bloodstream infections (BSIs) are related to high mortality and morbidity. Rapid administration of effective antimicrobial treatment is crucial for patient survival. Recently developed rapid methods to identify pathogens directly from blood culture bottles speed up diagnosis of BSIs. The present study compares the performance of two rapid identification methods, FilmArray and direct MALDI‐TOF MS, on identifying microorganisms directly from positive blood culture bottles with polymicrobial growth. FilmArray and direct MALDI‐TOF MS were performed directly on positive clinical and simulated polymicrobial blood culture bottles. Assay results were compared with standard culture methods. In total, 110 polymicrobial blood culture samples, of which 96 samples contained two microorganisms while 14 samples contained three microorganisms, were studied. FilmArray was able to identify 215/234 (92.0%) of isolates detected by the standard culture method and successfully identified all microorganisms in 88/110 (80.0%) of blood culture bottles. In contrast, direct MALDI‐TOF MS was only able to identify 65/234 (27.8%) of isolates and managed to identify all microoganisms in 2/110 (2.1%) of blood culture bottles. FilmArray is a rapid method for direct identification of polymicrobial blood culture samples that can complement the conventional identification methods. Direct MALDI‐TOF MS has low performance with polymicrobial samples.

中文翻译：

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直接从带有多菌生长的血培养瓶中鉴定微生物：FilmArray与直接MALDI-TOF MS的比较

血流感染（BSI）与高死亡率和高发病率有关。快速施用有效的抗菌治疗对患者生存至关重要。最近开发的快速方法可直接从血液培养瓶中鉴定病原体，从而加快BSI的诊断速度。本研究比较了两种快速识别方法FilmArray和直接MALDI-TOF MS在直接从具有阳性微生物培养的阳性血液培养瓶中鉴定微生物的性能。FilmArray和直接MALDI-TOF MS直接在阳性临床和模拟的微生物血液培养瓶上进行。将测定结果与标准培养方法进行比较。总共研究了110个多菌种血液培养物样本，其中96个样本包含两种微生物，而14个样本包含三种微生物。FilmArray能够鉴定通过标准培养方法检测到的215/234（92.0％）分离株，并成功鉴定了88/110（80.0％）血液培养瓶中的所有微生物。相比之下，直接MALDI-TOF MS仅能鉴定出65/234（27.8％）的分离株，并能鉴定出2/110（2.1％）的血培养瓶中的所有微生物。FilmArray是一种直接鉴定微生物血液培养物样本的快速方法，可以补充常规鉴定方法。直接MALDI-TOF MS对微生物样品的性能较低。8％的分离物，并设法鉴定出2/110（2.1％）血液培养瓶中的所有微生物。FilmArray是一种直接鉴定多菌种血液培养物样品的快速方法，可以补充传统的鉴定方法。直接MALDI-TOF MS对微生物样品的性能较低。8％的分离物，并设法鉴定出2/110（2.1％）血液培养瓶中的所有微生物。FilmArray是一种直接鉴定多菌种血液培养物样品的快速方法，可以补充传统的鉴定方法。直接MALDI-TOF MS对微生物样品的性能较低。