Phage therapy has been revitalized since antibiotic resistance in bacteria is increasing. Compared with antibiotics, phages can target specific bacteria precisely, which requires more understanding of phage-host interactions by investigating different phages. Escherichia coli is a common pathogen with very high diversity. Based on the O antigens, E. coli can be classified into at least 183 serotypes and existing phages are far from being able to lyse all E. coli. Therefore, a novel phage specific to E. coli, named DY1, was identified and characterized to enhance our understanding of the phages of E. coli and expand the phage library. Phage DY1 belongs to the family Autographiviridae which is derived from Podoviridae. The genome of DY1 was determined to be 39,817 bp and comprises 54 putative open reading frames. Comparative genome and phylogenetic analysis demonstrated that DY1 was highly similar to phages belonging to the genus Kayfunavirus; however, the highest average nucleotide identity (ANI) values of DY1 with known phages was 0.82 suggesting that DY1 was a novel phage. Through stability tests, DY1 was very stable at temperatures ranging from 20 to 50 °C and pH levels from 5 to 11. Taken together, we report that phage DY1 is a novel Kayfunavirus phage with the potential for phage therapy.

由于细菌对抗生素的耐药性正在增加，噬菌体疗法已经重新焕发生机。与抗生素相比，噬菌体可以精确靶向特定细菌，这需要通过研究不同的噬菌体来更多地了解噬菌体与宿主的相互作用。大肠杆菌是一种常见的病原体，具有非常高的多样性。根据O抗原，大肠杆菌至少可以分为183种血清型，现有的噬菌体还远不能裂解所有的大肠杆菌。因此，鉴定和表征了一种新的大肠杆菌特异性噬菌体DY1，以增强我们对大肠杆菌噬菌体的了解并扩大噬菌体文库。噬菌体DY1属于Autographiviridae家族它是从Podoviridae衍生的。DY1 的基因组被确定为 39,817 bp，包含 54 个推定的开放阅读框。比较基因组和系统发育分析表明 DY1 与属于Kayfunavirus属的噬菌体高度相似；然而，DY1与已知噬菌体的最高平均核苷酸同一性（ANI）值为0.82，表明DY1是一种新型噬菌体。通过稳定性测试，DY1 在 20 至 50 °C 的温度和 5 至 11 的 pH 值范围内非常稳定。综合起来，我们报告噬菌体 DY1 是一种具有噬菌体治疗潜力的新型Kayfunavirus噬菌体。