The recent renewal of cultural approach has substantially enriched knowledge of the human microbiota, notably through the discovery of new taxa from various anatomical sites. As an increasing number of these recent species are currently considered beneficial or harmful for human health, a constant updating of the repertoire of bacteria and archaea isolated from humans by culture is essential. Herein, we show that the number of cultured bacterial species associated with human beings increased, from 2776 in 2018, to 3253 in 2020, representing a 17% increase in 2 years by adding 477 species, of which 64% are new species (N = 307). A wide majority of the species added (i.e., 63%) were isolated using the culturomics approach, while 16% were cultured as part of clinical microbiology laboratories. Human microbiota studies would benefit from the completeness of the repertoire of bacteria associated with human beings, which would require continued efforts to culture microbes from human specimens.

最近文化方法的更新极大地丰富了人类微生物群的知识，特别是通过从各种解剖部位发现新的分类单元。由于目前认为这些新近物种对人体健康有益或有害，因此不断更新通过培养从人体内分离出的细菌和古细菌的种类至关重要。本文显示，与人类相关的培养细菌物种数量从2018年的2776种增加到2020年的3253种，通过添加477种在2年中增长了17％，其中64％是新物种（N = 307）。添加的大多数物种（即63％）使用文化学方法进行了分离，而16％的物种是作为临床微生物实验室的一部分进行培养的。