The global burden of cervical cancer from low and middle-income groups is increasing at alarming rates with more than half a million women being diagnosed every year. Although the disease is largely preventable when screened and diagnosed in earlier stages, the development of resistance and relapse had resulted in a poor prognosis. Therefore, a comprehensive approach needs to be put forward to understand and develop new preventive and therapeutic strategies to effectively combat cancer. Recently, much attention has been diverted to plant-derivatives for the treatment as they exhibit potent anti-cancer properties and side-effects caused by chemotherapeutic agents can also be prevented. Oleanolic acid and Esculetin are natural compounds known for their anti-cancer properties. Hence, the present study investigates the effect and mechanism of these compounds on cervical carcinoma, using HeLa cells. Posttreatment, it was observed that these compounds inhibited proliferation by both arresting the cells in the sub G1 phase and inducing senescence. Also, a marked reduction in the migration and cell survival was observed, as evidenced by results obtained from wound healing assay and Annexin V-FITC/PI staining. Furthermore, studies on the expression pattern of genes involved in major signaling pathways demonstrated a profound effect of these compounds. Taken together, the results of our study suggest that both Oleanolic acid and esculetin serve as a plausible therapeutic agent.

来自中低收入人群的子宫颈癌的全球负担正以惊人的速度增加，每年有超过50万妇女被诊断出患有这种疾病。尽管在早期阶段进行筛查和诊断时，该病在很大程度上是可以预防的，但耐药性和复发的发展导致预后不良。因此，需要提出一种全面的方法来理解和发展新的预防和治疗策略，以有效地对抗癌症。近来，由于植物衍生物显示出有效的抗癌特性并且还可以防止由化学治疗剂引起的副作用，因此已经将许多注意力转移到用于治疗的植物衍生物上。齐墩果酸和Esculetin是天然化合物，以其抗癌特性而闻名。因此，本研究使用HeLa细胞研究了这些化合物对宫颈癌的作用和机制。在后处理中，观察到这些化合物通过将细胞停滞在亚G1期并诱导衰老来抑制增殖。另外，从伤口愈合试验和膜联蛋白V-FITC / PI染色获得的结果证明，迁移和细胞存活率显着降低。此外，对涉及主要信号通路的基因表达模式的研究证明了这些化合物的深远影响。两者合计，我们的研究结果表明齐墩果酸和七叶皂甙都是可行的治疗剂。观察到这些化合物通过将细胞停滞在亚G1期并诱导衰老来抑制增殖。另外，从伤口愈合试验和膜联蛋白V-FITC / PI染色获得的结果证明，迁移和细胞存活率显着降低。此外，对涉及主要信号通路的基因表达模式的研究证明了这些化合物的深远影响。两者合计，我们的研究结果表明齐墩果酸和七叶皂甙都是可行的治疗剂。观察到这些化合物通过将细胞停滞在亚G1期并诱导衰老来抑制增殖。另外，从伤口愈合试验和膜联蛋白V-FITC / PI染色获得的结果证明，迁移和细胞存活率显着降低。此外，对涉及主要信号通路的基因表达模式的研究证明了这些化合物的深远影响。两者合计，我们的研究结果表明齐墩果酸和七叶皂甙都是可行的治疗剂。对涉及主要信号通路的基因表达模式的研究证明了这些化合物的深远影响。两者合计，我们的研究结果表明齐墩果酸和七叶皂甙都是可行的治疗剂。对涉及主要信号通路的基因表达模式的研究证明了这些化合物的深远影响。两者合计，我们的研究结果表明齐墩果酸和七叶皂甙都是可行的治疗剂。