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Effect of a short-term sodium chloride bath on juvenile pikeperch (Sander lucioperca) welfare
Aquaculture Reports ( IF 3.2 ) Pub Date : 2020-12-24 , DOI: 10.1016/j.aqrep.2020.100569
Krystyna Demska-Zakęś , Piotr Gomułka , Maciej Rożyński , Zdzisław Zakęś

This study aimed to determine the impact of immersing pikeperch (body weight approximately 94 g) in an aqueous solution of sodium chloride on gasometric, hematological, and biochemical indicators. The impact of the sodium chloride concentration (NaCl 10, 15, and 20 g L−1), immersion time (30 or 60 min) and time of blood sampling (0 h – blood drawn immediately following fish immersion; 24 h – blood drawn one full day after manipulation) was analyzed. The immersion protocol did not bring significant stress for tested fish as none of the tested parameters was different when compared to results obtained in a blank control group. The immersion times tested impacted the gasometric indicators and blood acid-base balance (0 h). The levels of most of the whole blood and plasma biochemical indicators analyzed (Na+, Cl-, glucose, lactate, total protein, globulin) differed significantly in fish exposed to 15 g L−1 and 20 g L−1 of NaCl from those in the blank control group. These changes were short-term and after 24 h the values of these indicators did not differ from those confirmed in the control group. Significant increases in the levels of hemoglobin, hematocrit, and red blood cells were confirmed in groups 10 g NaCl L−1/30 min/0 h and 20 g NaCl L−1/30 min/0 h and these changes persisted 24 h following immersion. Significant increases in the number of white blood cells (0 h) were confirmed in all groups, and this persisted for 24 h following immersion. The current study revealed transient, dose- and time-dependent stress reactions in pikeperch subjected to abrupt changes in water salinity and indicated that this species has significant recovery abilities in response to salinity shifts of short duration.



中文翻译:

短期氯化钠浴对幼体鲈(Sander lucioperca)福利的影响

这项研究的目的是确定将鲈鱼(体重约94 g)浸入氯化钠水溶液中对气体,血液和生化指标的影响。氯化钠浓度(NaCl 10、15和20 g L -1),浸泡时间(30或60分钟)和采血时间(0 h –鱼浸入后立即抽血; 24 h –采血)的影响操作一整天后进行了分析。与空白对照组相比,浸没方案不会给被测鱼带来很大的压力,因为被测参数没有任何不同。测试的浸入时间影响了气体指示剂和血酸碱平衡(0小时)。分析的大多数全血和血浆生化指标水平(Na+,氯- ,葡萄糖,乳酸盐,总蛋白,球蛋白)显著在鱼暴露到15克L-不同-1和20克L- -1从那些空白对照组中的NaCl。这些变化是短期的,24小时后这些指标的值与对照组确认的值没有差异。10 g NaCl L -1 / 30 min / 0 h和20 g NaCl L -1组的血红蛋白,血细胞比容和红细胞水平显着增加/ 30分钟/ 0小时,这些变化在浸入后24小时持续存在。在所有组中均确认白细胞数量显着增加(0小时),并且在浸泡后持续24小时。目前的研究表明,在盐度突然变化的情况下,鲈鱼的瞬时,剂量和时间依赖性胁迫反应,并表明该物种对短期盐度变化具有显着的恢复能力。

更新日期:2020-12-24
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