The objective of this study was to investigate the effects of 6 years of continuous biochar and biochar-based fertilizer (BBF) application on the soil bacterial community of brown earth soil (Cambisol) growing peanuts. There were five treatments: no fertilizer, low biochar (LB, 250 kg ha⁻¹), high biochar (HB, 750 kg ha⁻¹), chemical nitrogen (N)–phosphorus (P)–potassium (K) fertilizer (NPK, 83 kg ha⁻¹ N, 36 kg ha⁻¹ P, and 41 kg ha⁻¹ K), and BBF (750 kg ha⁻¹). The community richness (ACE) and community diversity (Shannon) indices were highest in the HB treatment, and the ACE of the HB treatment was higher than in the LB treatment (15.4 %). Proteobacteria was the dominant phylum (more than 38.5 % in all treatments). Compared to the CK and NPK treatments, Acidobacteria increased, and Actinobacteria decreased with biochar addition, but there were no significant differences in the microbial community structures of the biochar treatments (LB, HB, and BBF). The soil pH, total nitrogen, and available phosphorus were significantly associated with the bacterial community composition (phyla level). Cell wall/membrane/envelope biogenesis, signal transduction mechanisms, amino acid transport and metabolism, and energy production and conversion were the main gene types. The genes related to energy production and conversion, amino acid transport, metabolism, nucleotide transport and metabolism, carbohydrate transport and metabolism, and other functional roles were significantly more abundant in the biochar treatments (LB, HB, BBF) than in the CK and NPK treatments.

这项研究的目的是调查连续6年生物炭和生物炭基肥料（BBF）施用对种植花生的棕壤（Cambisol）的土壤细菌群落的影响。有五种处理方法：不施肥，低生物炭（LB，250 kg ha ^-1），高生物炭（HB，750 kg ha ^-1），化学氮（N）-磷（P）-钾（K）肥料（NPK） ，83 kg ha ^-1 N，36 kg ha ^-1 P和41 kg ha ^-1 K）和BBF（750 kg ha ^-1）。HB治疗的社区丰富度（ACE）和社区多样性（Shannon）指数最高，HB治疗的ACE高于LB治疗（15.4％）。变形细菌是占主导地位的门（在所有处理中均超过38.5％）。与CK和NPK处理相比，酸性细菌增加，放线菌增加随着添加生物炭而降低，但生物炭处理（LB，HB和BBF）的微生物群落结构没有显着差异。土壤的pH，总氮和有效磷与细菌群落组成（门叶水平）显着相关。细胞壁/膜/包膜的生物发生，信号转导机制，氨基酸转运和代谢以及能量产生和转化是主要的基因类型。在生物炭处理（LB，HB，BBF）中，与能量产生和转化，氨基酸转运，代谢，核苷酸转运和代谢，碳水化合物转运和代谢以及其他功能作用有关的基因比在CK和NPK中丰富得多。治疗。