Phosphorus (P) deficiency tolerance is a pivotal trait for plant growth and development. Most of the commercial modern cultivars lack this trait and reported it as a very serious problem limiting crop productivity. This trait is advantageous if present in modern high yielding varieties as it increases the yield under the phosphorus-deficient soil conditions. With the importance of phosphorus deficiency tolerance, the present investigation was carried out with an objective to screen for tolerance to phosphorus deficiency using solution culture and phosphorus uptake 1 (Pup1) locus linked markers in 30 diverse rice genotypes. A wide range of varied responses to P deficiency in rice genotypes for all the traits were observed. Root length and enzyme activity showed increased mean performance under the − P condition when compared to + P condition. Medium to high heritability estimates were obtained for most of the traits. Correlation analysis showed that the traits: root P content, fresh shoot weight, dry shoot weight, and shoot length showed highly significant correlations with each other under − P conditions. Based on the hydroponics and molecular screening, three genotypes viz., ADT (R) 48, Improved Pusa Basmati 1 and UPLRI 5 were classified as tolerant for its response to P deficiency as they possessed significant increase in desirable root and shoot traits, increased acid phosphatase enzyme and these genotypes also possessed the Pup1 allele for all the five markers. The selected genotypes may be useful for the exploration of novel genes conferring phosphorus deficiency tolerance and used as donor parents in the breeding programs. Absence of this allele in the rice genotypes viz., drought tolerant (Anna (R) 4) and submergence tolerant (CR 1009 Sub 1) may warrant the development of multiple abiotic stress tolerance cultivars for upland and submergence cropping systems in future rice breeding program.

磷（P）缺乏耐受性是植物生长发育的关键性状。大多数商业现代品种缺乏这一性状，并将其报告为限制作物生产力的一个非常严重的问题。如果该性状存在于现代高产品种中，则具有优势，因为它可以提高缺磷土壤条件下的产量。鉴于缺磷耐受性的重要性，本研究的目的是利用溶液培养和磷吸收 1 ( Pup1 ) 位点连锁标记在 30 种不同的水稻基因型中筛选缺磷耐受性。观察到水稻基因型的所有性状对磷缺乏的各种反应。与 + P 条件相比，- P 条件下的根长度和酶活性显示出平均性能有所提高。大多数性状都获得了中等到高的遗传力估计。相关分析表明，在-P条件下，根磷含量、鲜枝重、干枝重和枝长等性状之间呈现极显着相关。基于水培和分子筛选，三种基因型，即 ADT (R) 48、改良型 Pusa Basmati 1 和 UPLRI 5 被归类为对磷缺乏的耐受性，因为它们具有显着增加的所需根和芽性状、增加的酸度磷酸酶和这些基因型还拥有所有五个标记的Pup1等位基因。选定的基因型可能有助于探索赋予缺磷耐受性的新基因，并在育种计划中用作供体亲本。水稻基因型中不存在该等位基因。、耐旱（Anna (R) 4）和耐淹（CR 1009 Sub 1）可能需要在未来的水稻育种计划中为旱地和淹水种植系统开发多种非生物胁迫耐受品种。