The aim of this study was to screen the in vitro antimicrobial, antioxidant and cytotoxicity effect of Curkolin^®, a combination of curcumin and C. forskolin in the ratio 4:1. Curcuma longa L. is the source of curcumin while C. forskohlii has been used in traditional ayurvedic medicine for curing various disorders and therefore the forskolin is the source of the diterpenoids. The medicinal properties of plants are investigated throughout the world for scientific advancement for their important pharmacological activities, user convenient, economically viable with low toxicity. The antioxidant property of Curkolin® was determined to be 55.42 µg/ml using (Diphenyl picryl hydrazine) DPPH assay and was then compared with standard Butylated hydroxytoluene (BHT). The antimicrobial activity was assessed by calculating the MIC and MBC using microplate serial dilution technique. It exhibited 250 µg/ml, the least MIC value of Bacillus cereus as compared to the rest Gram-positive (Streptococcus mutans and Staphylococcus aureus) and Gram-negative (Escherichia coli and Salmonella typhi) bacteria. Further, it was analyzed for cytotoxic screening on MCF-7 breast cancer cell line by MTT assay, which has shown a strong anti-proliferative activity. The IC₅₀ values of Curkolin® were found 135.8 μg/ml. Together these results suggest the combination to be an effective antioxidant, antimicrobial and cytotoxic agent.

中文翻译：

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Curkolin®（长姜黄和锦紫苏forskohlii配方）的抗氧化，抗微生物和细胞毒性活性

本研究的目的是筛选体外Curkolin的抗微生物剂，抗氧化剂和细胞毒性效应^®的组合，姜黄素和C.毛喉素的比例为4:1。姜黄大号。C. forskohlii是姜黄素的来源，而C. forskohlii已在传统的印度草药疗法中用于治疗各种疾病，因此forskolin是二萜的来源。由于其重要的药理活性，方便用户使用，经济上可行且毒性低，在世界范围内对植物的药用特性进行了研究以寻求科学进步。Curkolin的抗氧化性能®用（二苯基苦基肼）DPPH测定法测得其含量为55.42 µg / ml，然后与标准丁基化羟基甲苯（BHT）进行比较。通过使用微孔板连续稀释技术计算MIC和MBC来评估抗菌活性。与其余的革兰氏阳性菌（变形链球菌和金黄色葡萄球菌）和革兰氏阴性菌（大肠埃希氏菌和 伤寒沙门氏菌）相比，它表现出250 µg / ml的最小蜡样芽胞杆菌MIC值。此外，通过MTT测定法对MCF-7乳腺癌细胞系进行了细胞毒性筛选，显示出很强的抗增殖活性。IC ₅₀ Curkolin的值®发现135.8微克/毫升。这些结果一起表明该组合是有效的抗氧化剂，抗微生物剂和细胞毒性剂。