Introduction: Pain and inflammatory disorders are the most prevalent syndromes. Different herbs were used for treatment of pain and inflammation including members of the genus Otostegia. As a result, this research investigated the in vivo analgesic and anti-inflammatory effects of 80% methanol leaf extract of Otostegia integrifolia in mice.
Methods: The analgesic and anti-inflammatory effects of the plant was evaluated using hot plate method, acetic-acid induced writhing test, and carrageenan and formalin induced paw edema. Three experimental groups (100, 200, and 400 mg/kg) received the extract while morphine 10 mg/kg and aspirin 150 mg/kg were used as a positive control for analgesic and anti-inflammatory tests accordingly. Distilled water (10 mL/kg) was used as negative control.
Results: From the experimental groups, OI400 displayed significant analgesic and anti-inflammatory activities (P< 0.001). In acetic acid induced writhing tests, the number of writhes decreased significantly (P< 0.001) in all experimental groups. Similarly, OI400 reduced the mean paw edema significantly in carrageenan and formalin induced paw edema (P< 0.05 and P< 0.001, respectively).
Conclusion: In general, the results obtained in this study demonstrated that the extract exhibited significant analgesic and anti-inflammatory potential in mice.

Keywords: analgesic activity, anti-inflammatory, hot plate, Otostegia integrifolia, carrageenan


中文翻译：

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Otostegia integrifolia Benth (Lamiaceae) 80% 甲醇叶提取物镇痛和抗炎潜力的评价

简介：疼痛和炎症性疾病是最普遍的综合征。不同的草药被用于治疗疼痛和炎症，包括耳痛属的成员。因此，本研究调查了80%整叶芫荽叶甲醇叶提取物在小鼠体内的镇痛和抗炎作用。方法：
采用热板法、醋酸致扭体试验、角叉菜胶和福尔马林致爪水肿等方法评价该植物的镇痛和抗炎作用。三个实验组（100、200 和 400 mg/kg）接受提取物，而吗啡 10 mg/kg 和阿司匹林 150 mg/kg 分别用作镇痛和抗炎试验的阳性对照。蒸馏水 (10 mL/kg) 用作阴性对照。
结果：从实验组来看，OI400表现出显着的镇痛和抗炎活性（P < 0.001）。在醋酸引起的扭体试验中，扭体次数显着减少（P< 0.001) 在所有实验组中。同样，OI400 显着降低了角叉菜胶和福尔马林诱导的爪水肿的平均爪水肿（分别为P < 0.05 和P < 0.001）。
结论：总的来说，本研究获得的结果表明，该提取物在小鼠中表现出显着的镇痛和抗炎潜力。

关键词:镇痛活性, 抗炎, 热板, Otostegia integrifolia , 角叉菜胶