Abstract A lncRNA RP1-85F18.6 was reported to affect cell growth by regulating the cell cycle. Here we tested whether it affects the proliferation of osteoblast cells by regulating the cell cycle. We determined the expression of RP1-85F18.6 in two osteoblast cell lines hFOB and HOB by qPCR. Then we knocked down or overexpressed RP1-85F18.6 in hFOB and tested the alteration of viability, cell cycle, and cell cycle regulatory proteins. Results showed that both hFOB and HOB expressed RP1-85F18.6. The knockdown of RP1-85F18.6 decreased the viability of hFOB, while the overexpression of it increased the viability. Higher expression of RP1-85F18.6 results in higher cell viability. The knockdown of RP1-85F18.6 caused an increase in the S phase cells and a decrease in the G2/M phase cells. The overexpression of RP1-85F18.6 caused a decrease in the S phase cells and an increase in the G2/M phase cells. The knockdown of RP1-85F18.6 decreased cyclin A, cdk1, E2F, cyclin B, p53, and p21, whereas the overexpression of RP1-85F18.6 increased cyclin A, cdk1, E2F, cyclin B, p53, and p21. This study demonstrated that RP1-85F18.6 is expressed in osteoblast cell lines hFOB and HOB. RP1-85F18.6 affects the proliferation of osteoblasts by regulating the cell cycle.

中文翻译：

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LncRNA RP1-85F18.6通过调节细胞周期影响成骨细胞

摘要 据报道，lncRNA RP1-85F18.6 通过调节细胞周期影响细胞生长。在这里我们测试了它是否通过调节细胞周期来影响成骨细胞的增殖。我们通过 qPCR 确定了 RP1-85F18.6 在两种成骨细胞系 hFOB 和 HOB 中的表达。然后我们在 hFOB 中敲低或过表达 RP1-85F18.6，并测试了活力、细胞周期和细胞周期调节蛋白的改变。结果表明，hFOB 和 HOB 均表达 RP1-85F18.6。RP1-85F18.6 的敲低降低了 hFOB 的活力，而它的过表达增加了活力。RP1-85F18.6 的更高表达导致更高的细胞活力。RP1-85F18.6 的敲低导致 S 期细胞的增加和 G2/M 期细胞的减少。RP1-85F18 的过度表达。6 导致 S 期细胞减少和 G2/M 期细胞增加。RP1-85F18.6 的敲低降低了细胞周期蛋白 A、cdk1、E2F、细胞周期蛋白 B、p53 和 p21，而 RP1-85F18.6 的过度表达增加了细胞周期蛋白 A、cdk1、E2F、细胞周期蛋白 B、p53 和 p21。该研究表明 RP1-85F18.6 在成骨细胞系 hFOB 和 HOB 中表达。RP1-85F18.6 通过调节细胞周期影响成骨细胞的增殖。