ABSTRACT

Anthocyanins are the main pigments in leaves, flowers and fruits, performing diverse biological functions in plants and exhibiting benefits for human health. The purple-leaved accession of Brassicacarinata was used to study the biosynthetic mechanism of anthocyanins in this study. To elucidate the mechanisms of anthocyanin accumulation in the purple-leaved line, we employed comparative transcriptome and iTRAQ-based proteome technique to investigate the expression profile of the purple-leaved line (BC-P01) in comparison with that of the green-leaved line (BC-G01) in B. carinata. In total, 58,124 transcripts and 4,631 proteins were identified, of which 5,183 and 285 exhibited significant changes in abundance between BC-G01 and BC-P01. Joint transcriptome and proteome analysis reveal six candidate genes of UDP-glucosyl transferase 79B1 (UGT79B1) for synthesis of cyanidin 3-O-xylosyl(1->2)glucoside, UDP-glucosyl transferase 79C1 (UGT75C1) for synthesis of cyanidin 3-O-xylosyl(1->2)glucoside, chalcone isomerase (CHI) and chalcone synthase (CHS) for synthesis of chalcone, anthocyanidin 5-O-glucoside-6„-O-malonyltransferase (AT5MAT) for malonylation of anthocyanins, granule bound starch synthase 1 (GBSS1) for amylose starch biosynthetic, glutathione s-transferase f3 (GSTF3) for encoding glutathione transferase for anthocyanin transporting, respectively. The expression level of the transcriptome data was further verified by qRT-PCR method. The study can provide useful information for biosynthesis of total anthocyanin content (TAC) and molecular breeding in B. carinata and other species in Brassiceae.

摘要

花青素是叶子，花朵和果实中的主要色素，在植物中执行多种生物学功能，并显示出对人类健康的益处。本研究以甘蓝型油菜紫叶为研究材料，研究了花色苷的生物合成机理。为了阐明花青素在紫叶线中的积累机制，我们采用了比较转录组和基于iTRAQ的蛋白质组技术，研究了紫叶线（BC-P01）与绿叶线相比的表达谱。 （B - carinata）（BC-G01）。总共鉴定出58,124个转录本和4,631个蛋白质，其中5,183和285个蛋白在BC-G01和BC-P01之间显示出丰度的显着变化。联合转录组和蛋白质组分析揭示了六个候选基因UDP葡糖基转移酶79B1（UGT79B1），用于矢车菊3-O-木糖基（1-> 2）葡糖苷，合成UDP葡糖基转移酶79C1（UGT75C1）为矢车菊3-O-合成-木糖基（1-> 2）葡萄糖苷，查尔酮异构酶（CHI）和查尔酮合酶（CHS）用于合成查尔酮，花色素苷5-O-葡萄糖苷-6„ -O-丙二酰转移酶（AT5MAT））对于花青素的丙二酰化作用，用于直链淀粉淀粉生物合成的颗粒结合淀粉合酶1（GBSS1），用于编码花青素转运的谷胱甘肽转移酶的谷胱甘肽s-转移酶f3（GSTF3）。转录组数据的表达水平通过qRT-PCR方法进一步验证。该研究可以提供总花色苷含量（TAC）和分子育种中的生物合成的有用的信息芥和其他物种中芸薹。