Abstract Context The natural products derived from Capparis ecuadorica H.H. Iltis (Capparaceae) could have great potential for anti-inflammation since they inhibited the inflammatory response in lipopolysaccharide (LPS)‐stimulated RAW 264.7 cells. Object This study investigated the anti-inflammatory effects and related mechanism of methanol extract of C. ecuadorica leaves (MCE) during atopic dermatitis (AD) responses. Materials and methods Alterations in the phenotypical markers for AD, luciferase signal, iNOS‐mediated COX‐2 induction pathway, and inflammasome activation were analysed in non-Tg (n = 5) and 15% phthalic anhydride (PA) treated IL-4/Luc/CNS-1 transgenic (Tg) HR1 mice (n = 5 per group), subsequent to treatment with acetone-olive oil (AOO), vehicle (DMSO) and two dose MCE (20 and 40 mg/kg) three times a week for 4 weeks. Results MCE treatment reduced the intracellular ROS level (48.2%), NO concentration (7.1 mmol/L) and inflammatory cytokine expressions (39.1%) in the LPS-stimulated RAW264.7 cells. A significant decrease was detected for ear thickness (16.9%), weight of lymph node (0.7 mg), IgE concentration (1.9 µg/mL), and epidermal thickness (31.8%) of the PA + MCE treated Tg mice. MCE treatment induced the decrease of luciferase signal derived from the IL-4 promoter and the recovery of the IL-4 downstream regulator cytokines. PA + MCE treated Tg mice showed decreasing infiltration of mast cells (42.5%), iNOS-mediated COX‐2 induction pathway, MAPK signalling pathway and inflammasome activation in the ear tissue. Conclusions These findings provide the first evidence that MCE may have great potential to suppress chemical-induced skin inflammation through the suppression of IL-4 cytokine and the iNOS-mediated COX‐2 induction pathway, and activation of inflammasome.

中文翻译：

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厄瓜多尔辣椒提取物对邻苯二甲酸酐诱导的 IL-4/Luc/CNS-1 转基因小鼠特应性皮炎的抗炎作用

摘要背景源自厄瓜多尔辣椒 HH Iltis (Capparaceae) 的天然产物具有巨大的抗炎潜力，因为它们可抑制脂多糖 (LPS) 刺激的 RAW 264.7 细胞的炎症反应。目的 本研究调查了厄瓜多尔桉叶 (MCE) 甲醇提取物在特应性皮炎 (AD) 反应中的抗炎作用和相关机制。材料和方法 在非 Tg (n = 5) 和 15% 邻苯二甲酸酐 (PA) 处理的 IL-4/ Luc/CNS-1 转基因 (Tg) HR1 小鼠（每组 n = 5），随后用丙酮-橄榄油 (AOO)、载体 (DMSO) 和两剂 MCE（20 和 40 mg/kg）治疗 3 次一周 4 周。结果 MCE处理降低了LPS刺激的RAW264.7细胞的细胞内ROS水平（48.2%）、NO浓度（7.1 mmol/L）和炎性细胞因子表达（39.1%）。检测到 PA + MCE 治疗的 Tg 小鼠的耳朵厚度 (16.9%)、淋巴结重量 (0.7 mg)、IgE 浓度 (1.9 µg/mL) 和表皮厚度 (31.8%) 显着降低。MCE 处理诱导源自 IL-4 启动子的荧光素酶信号减少和 IL-4 下游调节细胞因子的恢复。PA + MCE 处理的 Tg 小鼠显示肥大细胞浸润减少（42.5%）、iNOS 介导的 COX-2 诱导通路、MAPK 信号通路和耳组织炎症小体激活。