ABSTRACT

Zirconia is a bioinert ceramic biomaterial. Zirconia having composition of 97% Zirconia oxide and 3% Yttria oxide finds its vital application in the field of dental ceramics as an implant material for having good inert characteristics like minimum interaction with the adjacent tissues and exhibits good aesthetic property. This paper presents the in-vitro tests conducted to evaluation toxicity by cell culturing on zirconia biomaterial used in the dental implant by both direct contact and extraction method. In the present study, in-vitro assessment of tissue biocompatibility was conducted on L929 cell line (mouse fibroblast). In-vitro test, the toxicity of Zirconia specimen was done by computing percentage of viability in a cell-cultured medium. An MTT system was used to measure the active cell activities with mitochondrial dehydrogenases, which is an easy method which gives accurate and precision results. The results of biocompatibility in-vitro test by both Direct and Extraction methods confirmed that Zirconia exhibits the highest cell growth of 93.17% and resulted with zero-grade cytotoxicity. Zirconia having good aesthetic characteristics, i.e. colour of the implant matches with the tooth colour. Hence Zirconia is a candidate implant material than other metal implants.

摘要

氧化锆是一种生物惰性陶瓷生物材料。具有97％的氧化锆和3％的氧化钇组成的氧化锆作为具有良好惰性特性（例如与相邻组织的相互作用最小）的植入材料而在牙科陶瓷领域中得到了重要应用，并表现出良好的美学特性。本文介绍了体外测试，通过直接接触法和拔牙法对在种植牙中使用的氧化锆生物材料进行细胞培养来评估毒性。在本研究中，在L929细胞系（小鼠成纤维细胞）上进行了组织生物相容性的体外评估。体外测试中，氧化锆样品的毒性通过计算在细胞培养基中的生存力百分比来确定。使用MTT系统通过线粒体脱氢酶测量活性细胞的活性，这是一种简便易行的方法，可提供准确而精确的结果。通过直接和提取方法进行的体外生物相容性测试结果证实，氧化锆显示出最高的细胞生长率为93.17％，并导致零级细胞毒性。氧化锆具有良好的美学特性，即植入物的颜色与牙齿的颜色相匹配。因此，氧化锆比其他金属植入物是候选的植入物材料。