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A redundant hydraulic function of root hairs in barley plants grown in hydroponics
Functional Plant Biology ( IF 3 ) Pub Date : 2020-12-22 , DOI: 10.1071/fp20287
Shannon Burke , Emma Sadaune , Lisa Rognon , Alexane Fontana , Marianne Jourdrin , Wieland Fricke

The root hair-less brb of Hordeum vulgare L. (bald root barley) mutant was used to assess the significance that root hairs have for the hydraulic properties of roots and response to a limited supply of mineral nutrients in plants grown on hydroponics. The barley brb mutant and its parent wild-type (H. vulgare cv. Pallas) were grown under nutrient sufficient control conditions, and under conditions of low supply of P and N. Plants were analysed when they were 14–18 days old. Root hydraulic conductivity (Lp) was determined for excised root systems and intact transpiring plants, and cell Lp was determined through cell pressure probe measurements. The formation of Casparian bands and suberin lamellae was followed through staining of cross-sections. The presence or absence of root hairs had no effect on the overall hydraulic response of plants to nutritional treatments. Root and cell Lp did not differ between the two genotypes. The most apparent difference between brb and wild-type plants was the consistently reduced formation of apoplastic barriers in brb plants. Any hydraulic function of root hairs can be redundant in barley, at least under the hydroponic conditions tested.



中文翻译:

水培法栽培大麦植物根毛的多余水力功能

根毛发较少BRB大麦L.(秃根大麦)突变体被用来评估根毛具有用于根和响应的矿物营养物有限的供应在植物生长在水栽液压特性的意义。大麦brb突变体及其亲本野生型(H. vulgare简历。帕拉斯(Pallas)在营养充足的控制条件下生长,并在磷和氮供应量低的条件下生长。对植株生长14–18天时进行了分析。确定切除的根系和完整转运植物的根水力传导率(Lp),并通过细胞压力探针测量确定细胞Lp。通过横截面染色来追踪里海带和木栓层的形成。根毛的有无对植物对营养处理的总体水力响应没有影响。两种基因型之间的根和细胞Lp没有差异。之间的最明显的区别BRB和野生型植物是在质外体屏障的一致减少形成BRB植物。至少在测试的水耕条件下,大麦根毛的任何水力功能都是多余的。

更新日期:2020-12-23
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