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Taxonomic revisions based on genetic analysis of type specimens of Ulva conglobata, U. laetevirens, U. pertusa and U. spathulata (Ulvales, Chlorophyta)
Phycological Research ( IF 1.6 ) Pub Date : 2020-12-23 , DOI: 10.1111/pre.12450
Jeffery R. Hughey 1 , Paul W. Gabrielson 2 , Christine A. Maggs 3, 4 , Frédéric Mineur 3 , Kathy Ann Miller 5
Affiliation  

Genetic analysis of type material of various Ulva species has shown that many names have been misapplied. DNA sequences of two of the specimens on the lectotype sheet of U. australis from South Australia showed that U. australis is conspecific with specimens called U. pertusa from Japan. However, type material of U. pertusa has not been sequenced, nor have other type specimens with type localities in Japan and Australia, including U. conglobata f. conglobata and U. conglobata f. densa from the former, and U. laetevirens and U. spathulata from the latter. To determine if these names have been correctly applied, targeted DNA sequencing of the rbcL gene was performed on (i) the lectotype specimen of U. laetevirens, (ii) the lectotype and two syntype specimens of U. pertusa, (iii) the lectotype and two syntype specimens of U. congoblata f. conglobata and (iv) two specimens on the holotype sheet of U. conglobata f. densa. In addition, high‐throughput sequencing (HTS) analysis was carried out on an isotype specimen of U. spathulata. Phylogenetic analysis of the resulting rbcL gene sequences supports the taxonomic conclusions that U. laetevirens, U. pertusa and U. spathulata are synonyms of U. australis, and U. conglobata f. conglobata and U. conglobata f. densa represent a single separate and distinct species, U. conglobata. These genetic data contribute to the taxonomy of Ulva and further demonstrate that analyzing DNA from type material is the only currently known reliable method to correctly apply names in this genus.

中文翻译:

基于对Ulva conglobata,U。laetevirens,U。pertusa和U. spathulata(Ulvales,Chlorophyta)的类型标本进行遗传分析的分类学修订

各种Ulva种类的类型材料的遗传分析表明,许多名称被错误地使用。两个上的选型片样品的DNA序列U.芦苇从南澳大利亚表明U.芦苇是同种同标本称为孔石莼来自日本。然而,没有对百日草的类型标本进行测序,在日本和澳大利亚也没有对具有地方类型的其他类型标本进行测序,其中包括美国卷柏(U. conglobata f。)。conglobatau。conglobata f。前者的densa,以及U. laetevirensU. spathulata从后者。要确定是否这些名字已被正确地应用,所述的靶向DNA测序RBC L基因被执行(i)的样本选型U. laetevirens,(ii)所述选型和两个共型标本孔石莼,(iii)所述刚果U.的选型和两个同型标本。conglobata和(iv)conglobata f。完整版表上的两个标本。丹莎。此外,高通量测序(HTS)分析在的同种型样品进行U.匙叶。所产生的rbc L基因序列的系统发育分析支持以下分类学结论:U. laetevirensU。pertusaU. spathulataU. australisU. conglobata f。的同义词。conglobatau。conglobata f。致密表示单个单独且不同的品种,U.聚合性。这些遗传数据有助于Ulva的分类,并进一步证明分析类型材料中的DNA是目前唯一正确应用此属名称的可靠方法。
更新日期:2020-12-23
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