The Japanese apogamous fern Dryopteris yakusilvicola was previously thought to be a hybrid of D. sabaei and D. sparsa var. sparsa based on morphological characterization and enzyme electrophoresis. However, the phylogenetic relationships with D. hayatae, D. melanocarpa var. melanocarpa, D. melanocarpa var. elegans, and D. sparsa var. ryukyuensis have been unclear. We performed a molecular phylogenetic analysis of the DNA sequences of two plastid (trnL‐F, rbcL) and one low‐copy nuclear marker (AK1) of Dryopteris yakusilvicola and related species. The origin of D. yakusilvicola is not a hybrid between D. sparsa var. sparsa and D. sabaei, but the parents appear to have been undetected species closely related to D. melanocarpa var. elegans and D. sabaei. Analysis of additional species of the D. sparsa complex around Southeast Asia and additional nuclear markers are needed to clarify the relationships of this complex.

中文翻译：

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无融合生日本蕨Dryopteris yakusilvicola（Dryopteridaceae）的起源

以前人们认为日本无融合生蕨Dryopteris yakusilvicola是D. sabaei和D. sparsa var的杂种。基于形态表征和酶电泳的稀疏。然而，与D. hayatae，D。melanocarpa var的系统发育关系。黑色果皮，D. melanocarpa var。线虫和D. sparsa var。ryukyuensis尚不清楚。我们对两种质体（trnL-F，rbcL）和一种低拷贝核标记（鳞翅目夜蛾和相关物种的AK1）。D. yakusilvicola的起源不是D. sparsa var之间的杂种。sparsa和D. sabaei，但其亲本似乎是未发现的与D. melanocarpa var密切相关的物种。线虫和D. sabaei。需要分析东南亚周围的D. sparsa复合体的其他物种和其他核标记，以阐明该复合体的关系。