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Corrigendum: PasT of Escherichia coli sustains antibiotic tolerance and aerobic respiration as a bacterial homolog of mitochondrial Coq10
MicrobiologyOpen ( IF 3.9 ) Pub Date : 2020-12-22 , DOI: 10.1002/mbo3.1140


This article corrects the following:

Cinzia Fino, Martin Vestergaard, Hanne Ingmer, Fabien Pierrel, Kenn Gerdes, Alexander Harms

Correction regarding Escherichia coli O157:H7 strain EDL933

When revisiting the different enterobacterial strains used in our article (Fino et al., 2020), we discovered that Escherichia coli O157:H7 strain EDL933 had been misidentified. As outlined in the original article by Marinus and Poteete (2013), the strain identified as GM9255 is not a mutant of the original EDL933 strain in which the Shiga toxin genes encoded in the 933w prophage have been inactivated (which would be GM9251), but instead an E. coli K‐12 derivative that had been lysogenized with this engineered prophage. The results presented in appendix figures A3, A5, and A6 with strain GM9255—identified as “E. coli O157:H7 EDL933”—are therefore not informative about the biology of PasTI in an E. coli O157:H7 strain. Due to the close relationship of RatAB/PasTI in all organisms used in our work, we did not recognize this misidentification when constructing the ΔpasTI mutant of the strain that we thought was E. coli O157:H7 EDL933. This misidentification does not affect any conclusions of our work, because all data shown in the main figures have been generated with our primary model organisms Escherichia coli CFT073 and Escherichia coli K‐12 MG1655. The strain that we thought was E. coli O157:H7 EDL933 has only been indicated in appendix figures A3, A5, and A6 to demonstrate the general relevance of our findings for enterobacteria beyond our model organisms. For this purpose, the data generated with this strain were always paired with comparable data generated with pathogenic E. coli strain 55989 and Salmonella enterica strain SR‐11 that yielded similar results. The authors apologize for this oversight.



中文翻译:

更正:大肠杆菌的PasT作为线粒体Coq10的细菌同源物,维持抗生素耐受性和有氧呼吸

本文纠正了以下问题:

Cinzia Fino,Martin Vestergaard,Hanne Ingmer,Fabien Pierrel,Kenn Gerdes,Alexander Harms

关于大肠杆菌O157:H7菌株EDL933的更正

在回顾本文中使用的不同肠杆菌菌株时(Fino等人,2020年),我们发现大肠杆菌O157:H7菌株EDL933被误认了。如Marinus和Poteete(2013)的原始文章所述,鉴定为GM9255的菌株不是原始EDL933菌株的突变体,在原始EDL933菌株中,933w噬菌体中编码的志贺毒素基因已失活(将为GM9251),但代替ê。已被该工程原 菌裂解的大肠杆菌K-12衍生物。附录图A3,A5和A6中显示的结果为菌株GM9255(标识为“ E”) 大肠杆菌O157:H7 EDL933”,因此无法提供有关E中PasTI生物学的信息。 大肠杆菌O157:H7菌株。由于RatAB / PasTI与我们工作中使用的所有生物都有密切的关系,因此在构建我们认为是E的菌株的ΔpasTI突变体时,我们没有意识到这种错误识别。 大肠杆菌O157:H7 EDL933。这种错误识别不会影响我们的工作任何结论,因为主要数据中显示的所有数据都是使用我们的主要模式生物大肠杆菌CFT073和大肠杆菌K-12 MG1655生成的。我们认为该菌株是Ë 大肠杆菌O157:H7 EDL933仅在附录图A3,A5和A6中指出,以证明我们的发现与我们模型生物之外的肠道细菌的普遍相关性。为此,该菌株产生的数据总是与致病性E产生的可比数据配对。 大肠杆菌55989菌株和肠沙门氏菌SR-11菌株产生了相似的结果。作者对此表示歉意。

更新日期:2020-12-23
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