Erythrocyte transketolase activity coefficient (ETKAC) assay protocol for the assessment of thiamine status,Annals of the New York Academy of Sciences

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Erythrocyte transketolase activity coefficient (ETKAC) assay protocol for the assessment of thiamine status
Annals of the New York Academy of Sciences ( IF 4.1 ) Pub Date : 2020-12-22 , DOI: 10.1111/nyas.14547
Kerry S Jones ₁ , Damon A Parkington ₁ , Lorna J Cox _{1,

2} , Albert Koulman ₁

Affiliation

Vitamin B1 (thiamine) is an essential nutrient that acts as a cofactor for a number of metabolic processes, particularly in energy metabolism. Symptoms of classic thiamine deficiency are recognized as beriberi, although clinical symptoms are nonspecific and recognition of subclinical deficiency is difficult. Therefore, reliable biomarkers of thiamine status are required. Thiamine diphosphate is a cofactor for transketolase, including erythrocyte transketolase (ETK). The ETK activity assay as an indirect, functional marker of thiamine status has been used for over 50 years. The ETK activity assay provides a sensitive and specific biomarker of thiamine status; however, there is a lack of consensus over the cutoffs for deficiency, partly due to a lack of assay harmonization. Here, we provide a step-by-step protocol for the measurement of ETK activity and the calculation of the ETK activity coefficient, including detailed explanations of equipment and chemicals required and guidance for quality control procedures. Harmonization of the protocol will provide the basis for the development of internationally recognized cutoffs for thiamine insufficiency. The establishment of quality control materials and a quality assurance scheme are recommended to provide reliability. This will ensure that the ETK activity assay remains an important method for the assessment of thiamine status.

中文翻译：

用于评估硫胺素状态的红细胞转酮酶活性系数 (ETKAC) 测定方案

维生素 B1（硫胺素）是一种必需营养素，可作为许多代谢过程（特别是能量代谢）的辅助因子。典型的硫胺素缺乏症的症状被认为是脚气病，尽管临床症状是非特异性的，并且很难识别亚临床缺乏症。因此，需要可靠的硫胺素状态生物标志物。二磷酸硫胺素是转酮酶的辅助因子，包括红细胞转酮酶 (ETK)。 ETK 活性测定作为硫胺素状态的间接功能标记，已使用了 50 多年。 ETK 活性测定提供了硫胺素状态的敏感且特异的生物标志物；然而，对于缺陷的临界值缺乏共识，部分原因是缺乏检测协调。在这里，我们提供了测量 ETK 活性和计算 ETK 活性系数的分步方案，包括所需设备和化学品的详细说明以及质量控制程序的指导。该议定书的协调将为制定国际公认的硫胺素缺乏临界值奠定基础。建议建立质量控制材料和质量保证方案以提供可靠性。这将确保 ETK 活性测定仍然是评估硫胺素状态的重要方法。

更新日期：2020-12-22

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