In this paper we report successful integration of digital holography in off- axis transmission configuration into a commercially available pathology microscope (PathologyDHM), where Mach-Zehnder interferometric configuration was achieved in pathology microscope using 1 × 2 single mode fiber coupler and custom built optical modules. A smartphone/webcam positioned at the image plane of the finite conjugate microscope captures the interference pattern generated by the system. Using PathologyDHM, we have demonstrated a novel and simple method for compensation of aberration due to microscope objective (MO). The proposed method is called defocus hologram method and it is particularly useful in cases, where samples do not have specimen free or background flat region. Proper validation of the method was done by imaging a transmissive grating (commercial compact disc, CD-R), which doesn't have a flat background region and comparing phase profile of CD-R computed by our proposed method with self-reference conjugated hologram method. For reiterating its biological applications, phase profile of a biological sample, red blood cell smear (RBC smear), was computed with our defocus hologram method and compared with already established reference conjugated hologram method. Phase profiles of CD-R and RBC smear computed with defocus hologram method agree with self-reference and reference conjugated hologram methods respectively, within the phase error limits of the system. Integration of digital holography in to commercial pathology microscope enables 3D imaging, which coupled with artificial intelligence may prove useful in disease diagnostics and monitoring response of cells to treatment.

在本文中，我们报告了将离轴传输配置中的数字全息技术成功集成到市售的病理显微镜（PathologyDHM）中，其中使用1×2单模光纤耦合器和定制光学模块在病理显微镜中实现了Mach-Zehnder干涉配置。放置在有限共轭显微镜像面上的智能手机/网络摄像头捕获系统产生的干涉图。使用PathologyDHM，我们展示了一种新颖且简单的方法来补偿由于显微镜物镜（MO）引起的像差。所提出的方法称为散焦全息图方法，在样品没有无标本或背景平坦区域的情况下特别有用。该方法的正确验证是通过对不具有平坦背景区域的透射光栅（商业光盘，CD-R）进行成像，然后将我们提出的方法计算出的CD-R的相位分布与自参考共轭全息图进行比较方法。为了重申其生物学应用，使用我们的散焦全息图方法计算了生物样品的相图，即红细胞涂片（RBC涂片），并与已经建立的参考共轭全息图方法进行了比较。用散焦全息图方法计算的CD-R和RBC涂片的相位分布分别在系统的相位误差范围内分别与自参考和参考共轭全息图方法一致。将数字全息技术集成到商业病理显微镜中可实现3D成像，