This paper focuses on developing a sustainable and integrated process for the biocatalytic extraction of sinapic acid from the waste biomass of oleaginous “oil-bearing” seeds. Using an optimized chemo-enzymatic technique, sinapine was effectively liberated from mustard bran and then completely hydrolyzed into sinapic acid. Several parameters were optimized to release sinapic acid (e.g., mustard species, extraction methods, mustard production dates, ethanol concentration, solids loading, extraction time and enzyme type or concentration). Subsequently, an integrated bioprocess was developed and scaled up guided. The results revealed that the biomass type or source and the enzyme used can substantially impact the release of sinapic acid, and overall cost, respectively. For the Canadian lot of crude mustard bran, ~10 mg of sinapic acid per g mustard bran was successfully extracted, along with 43 mg total phenolics per g mustard bran and 71 mg sugars per g mustard bran. Lastly, supplementary portions of the biomass (i.e., lipids) were also discussed for their applicability as value added products.

本文着重于开发一种可持续的综合方法，用于从油脂性“含油”种子的废弃生物质中生物催化提取芥子酸。使用优化的化学酶促技术，芥子碱可以有效地从芥菜麸中释放出来，然后完全水解为芥子酸。优化了几个参数以释放芥子酸（例如，芥末种类，提取方法，芥末生产日期，乙醇浓度，固体含量，提取时间和酶类型或浓度）。随后，开发了一个集成的生物过程并扩大了规模。结果表明，生物质的类型或来源以及所用的酶可分别显着影响芥子酸的释放和总成本。对于加拿大的大量芥末糠，成功提取了每克芥末糠约10毫克芥子酸，每克芥末糠中总酚43毫克和每克芥末糠中有71毫克糖。最后，还讨论了生物质的补充部分（即脂质）作为增值产品的适用性。