Lignin, a complex aromatic polymer, is a structural component of plant biomass. It decomposes with difficulty because of its rigidity properties, however, lignin valorization is essential for the economics of lignocellulosic biorefineries. Pseudomonas putida has been extensively investigated as a promising host strain for lignin valorizations due to intrinsic traits, such as low nutritional requirement, high tolerance to toxicity, and metabolic versatility with a wide spectrum of substrates, such as aromatic compounds. Although a naturally occurring, lignin-utilizing P. putida strain has been reported, it is necessary to engineer the genome of P. putida for efficient lignin utilization. For biological lignin valorization, the decomposition of lignin polymer to low-molecular weight compounds and transformation of lignin-derived aromatic compounds to value-added chemicals is essential. Various tools of synthetic biology have been developed for the genome engineering of P. putida; efforts in metabolic engineering have been made to expand aromatic substrate specificity and to improve productivity of value-added chemicals. Development of high-performance bio-parts and biosensors for lignin valorization has also been done. In this review, we present recent research on genome engineering tools developed for P. putida and metabolic engineering employed in P. putida to improve lignin valorization.

木质素是一种复杂的芳香聚合物，是植物生物量的结构成分。由于其刚性性质，其难以分解，但是，木质素的价衡对于木质纤维素生物精炼厂的经济性是必不可少的。恶臭假单胞菌已被广泛研究为有前景的木质素增价宿主菌株，这是由于其固有特性，例如营养需求低，对毒性的高耐受性以及具有多种底物（例如芳香族化合物）的代谢多功能性。尽管已经报道了天然存在的利用木质素的恶臭假单胞菌菌株，但是有必要对恶臭假单胞菌的基因组进行改造。高效利用木质素。对于生物木质素增值，木质素聚合物分解为低分子量化合物以及木质素衍生的芳族化合物转化为增值化学品至关重要。已经开发了多种合成生物学工具用于恶臭假单胞菌的基因组工程; 已经进行了代谢工程方面的努力，以扩大芳香族底物的特异性并提高增值化学品的生产率。木质素增值的高性能生物部件和生物传感器的开发也已经完成。在这次审查中，我们提出了近期对开发基因工程工具的研究恶臭假单胞菌和使用的代谢工程恶臭假单胞菌，提高木质素稳定物价。