Liposomes are well studied and proved to have enormous potential for carboplatin delivery in cancer treatment. However, to achieve an ideal liposome system with less leakage and controlled release for an effective delivery of carboplatin (CPT) to tumor sites is still in progress. In this study, poly(ethylene glycol) methyl ether-cholesterol (mPEG-Chol) was prepared and used as a component, together with soy lecithin and cholesterol, to synthesize liposomes through thin film hydration method. The particle size distribution of obtained system of mPEG-Chol modified soy lecithin liposomes (mPEG-Chol-SLP) was then reduced by sonication, followed by extrusion. The properties of these nanoparticles including particle size, polydispersity index, zeta potential, and morphology were assessed by dynamic light scattering (DLS), zeta potential measurement, and transmission electron microscopy (TEM). Using dialysis method as the in vitro tests, the drug loading and releasing efficiency were calculated with pre-determined formulas. The cytotoxicity in the human breast cancer cell line (MCF-7) was evaluated through the cell proliferation assay WST-1. The results showed that CPT-loaded mPEG-Chol-SLP was spherical in shape with the average particle diameter and polydispersity index of 173.10 nm and 0.174, respectively. Moreover, the system was negatively charged, relatively colloidal stable, 70.49% for drug loading efficiency, and slowly released up to 48 h. In addition, the cytotoxicity data showed that mPEG-Chol-SLP was biocompatible nanocarrier and successfully reduced the toxicity of CPT. These results demonstrated that the obtained CPT-loaded mPEG-Chol-SLP reached one step closer to an ideal nanocarrier for CPT delivery.

脂质体已经过充分研究，并被证明在卡铂治疗癌症中具有巨大的潜力。然而，实现具有较少泄漏和控制释放以将卡铂（CPT）有效递送至肿瘤部位的理想脂质体系统仍在进行中。在这项研究中，制备了聚（乙二醇）甲基醚-胆固醇（mPEG-Chol），并与大豆卵磷脂和胆固醇一起用作成分，通过薄膜水化法合成脂质体。然后通过超声处理，然后挤出，减小获得的mPEG-Chol修饰的大豆卵磷脂脂质体系统（mPEG-Chol-SLP）的粒度分布。通过动态光散射（DLS），ζ电位测量，评估了这些纳米粒子的性质，包括粒径，多分散指数，ζ电位和形态。和透射电子显微镜（TEM）。使用透析方法作为在体外试验中，药物载量和释放效率用预定公式计算。通过细胞增殖测定WST-1评估人乳腺癌细胞系（MCF-7）中的细胞毒性。结果表明，载有CPT的mPEG-Chol-SLP为球形，平均粒径和多分散指数分别为173.10 nm和0.174。此外，该系统带负电，相对胶体稳定，载药效率为70.49％，并在48小时内缓慢释放。此外，细胞毒性数据表明，mPEG-Chol-SLP是生物相容性纳米载体，并成功降低了CPT的毒性。这些结果证明，所获得的CPT负载的mPEG-Chol-SLP比用于CPT递送的理想纳米载体更近一步。