Flow diversion aims at treatment of intracranial aneurysms via vessel remodeling mechanisms, avoiding the implantation of foreign materials into the aneurysm sack. However, complex implantation procedure, high metal surface and hemodynamic disturbance still pose a risk for thromboembolic complications in the clinical praxis. A novel fibrin and heparin based nano coating considered as a hemocompatible scaffold for neointimal formation was investigated regarding thrombogenicity and endothelialization. The fibrin-heparin coating was compared to a bare metal as well as fibrin- or heparin-coated flow diverters. The implants were tested separately in regard to inflammation and coagulation markers in two different in vitro hemocompatibility models conducted with human whole blood (n = 5). Endothelialization was investigated through a novel dynamic in vitro cell seeding model containing primary human cells with subsequent viability assay. It was demonstrated that platelet loss and platelet activation triggered by presence of a bare metal stent could be significantly reduced by applying the fibrin-heparin, fibrin and heparin coating. Viability of endothelial cells after proliferation was similar in fibrin-heparin compared to bare metal implants, with a slight, non-significant improvement observed in the fibrin-heparin group. The results suggest that the presented nanocoating has the potential to reduce thromboembolic complications in a clinical setting. Though the new model allowed for endothelial cell proliferation under flow conditions, a higher number of samples is required to assess a possible effect of the coating.

分流旨在通过血管重塑机制治疗颅内动脉瘤，避免异物植入动脉瘤袋。然而，复杂的植入过程、高金属表面和血流动力学紊乱在临床实践中仍然存在血栓栓塞并发症的风险。研究了一种新型纤维蛋白和肝素纳米涂层，该涂层被认为是新内膜形成的血液相容性支架，研究了血栓形成和内皮化。将纤维蛋白-肝素涂层与裸金属以及纤维蛋白或肝素涂层的分流器进行比较。在用人全血进行的两种不同体外血液相容性模型中，分别对植入物的炎症和凝血标志物进行了测试（n= 5）。通过新的体外动态研究内皮化包含原代人类细胞的细胞接种模型以及随后的活力测定。结果表明，通过应用纤维蛋白-肝素、纤维蛋白和肝素涂层，可以显着减少由裸金属支架的存在引发的血小板损失和血小板活化。与裸金属植入物相比，纤维蛋白-肝素组内皮细胞增殖后的存活率相似，但在纤维蛋白-肝素组中观察到轻微的非显着改善。结果表明，所提出的纳米涂层有可能减少临床环境中的血栓栓塞并发症。虽然新模型允许在流动条件下内皮细胞增殖，但需要更多的样本来评估涂层的可能影响。