The purpose of this study was to examine the impact of hydrophobicity, surface chemistry and topography on bacterial and microalgal adhesion. To this end, the effects of surface plasma treatments (argon (Ar) and tetrafluorocarbon (CF₄)) of polycarbonate substrates on bioadhesion were investigated in vitro. Surfaces were characterized by goniometry, atomic force microscopy and X-ray photoelectron spectroscopy. Two marine bacterial strains, a hydrophobic Pseudoalteromonas sp. 5M6 and a hydrophilic Paracoccus sp. 4M6, and two microalgae (Cylindrotheca closterium and Porphyridium purpureum) involved in marine biofouling were selected. Their adhesion onto the substrates was observed and quantified using confocal laser scanning microscopy–microfluidic flow cells. It was demonstrated that the combination of three parameters – namely, surface energy, fluorination and nanotopography – significantly decreases the adhesion of three microorganisms out of four (Pseudoalteromonas sp. 5M6, C. closterium and P. purpureum), whereas one parameter on its own is insufficient.

中文翻译：

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表面等离子体处理（Ar / CF4）减少了聚碳酸酯表面的生物污垢

这项研究的目的是研究疏水性，表面化学和形貌对细菌和微藻粘附的影响。为此，在体外研究了聚碳酸酯基材的表面等离子体处理（氩气（Ar）和四氟化碳（CF ₄））对生物粘附的影响。通过测角法，原子力显微镜和X射线光电子能谱对表面进行了表征。两种海洋细菌菌株，一种疏水性假单胞菌属sp。5M6和亲水性Paracoccus sp。4M6和两个微藻（Cylindrotheca closterium和Porphyridium purpureum）选择参与海洋生物污染的船舶。使用共聚焦激光扫描显微镜–微流体流通池观察并定量了它们在基材上的附着力。结果表明，表面能，氟化和纳米形貌这三个参数的组合显着降低了四种微生物（假单孢菌5M6，梭状芽胞杆菌和紫脓杆菌）中三种微生物的粘附力，而一个参数本身不足。