Fluorescent-Raman Binary Star Ratio Probe for MicroRNA Detection and Imaging in Living Cells,Analytical Chemistry

当前位置： X-MOL 学术 › Anal. Chem. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Fluorescent-Raman Binary Star Ratio Probe for MicroRNA Detection and Imaging in Living Cells
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-12-21 , DOI: 10.1021/acs.analchem.0c03491
Jihua Zhang ₁ , Hao Zhang ₂ , Sujuan Ye ₁ , Xingxiang Wang ₁ , Lindong Ma ₁

Affiliation

The expression of microRNAs (miRNAs) is critical in gene regulation and has been counted into disease diagnosis marks. Precise imaging and quantification of miRNAs could afford the important information for clinical diagnosis. Here, two smart binary star ratio (BSR) probes were designed and constructed, and miRNA triggered the connection of the binary star probes and the reciprocal changes of dual signals in living cells. This multifunctional probe integrates fluorescence and surface enhanced Raman scattering (SERS) imaging, with enzyme-free numerator signal amplification for dual-mode imaging and dual-signal quantitative analysis of miRNA. First, compared with the single-mode ratio imaging method, using fluorescence-SERS complementary ratio imaging, this probe enables more accurate imaging contrast for direct visualization signal changes in living cells. Multiscale information about the dynamic behavior of miRNA and the probe is acquired. Next, via SERS reverse signal ratio response and a novel enzyme-free numerator signal amplification, the amplified signal and reduced black value were achieved in the quantification of miRNA. More importantly, BSR probes showed good stability in cells and were successfully used for accurate tracing and quantification of miR-203 from MCF-7 cells. Therefore, the reported BSR probe is a potential tool for the reliable monitoring of biomolecule dynamics in living cells.

中文翻译：

用于活细胞中MicroRNA检测和成像的荧光拉曼二元星比探针

microRNA（miRNA）的表达在基因调控中至关重要，已被计入疾病诊断标记。miRNA的精确成像和定量分析可为临床诊断提供重要信息。在这里，设计并构建了两个智能双星比率（BSR）探针，miRNA触发了双星探针的连接以及活细胞中双信号的倒数变化。这款多功能探针将荧光和表面增强拉曼散射（SERS）成像与无酶分子信号放大集成在一起，用于miRNA的双模式成像和双信号定量分析。首先，与单模比率成像方法相比，使用荧光SERS互补比率成像，该探头可实现更准确的成像对比度，以便直接可视化活细胞中的信号变化。获取有关miRNA和探针动态行为的多尺度信息。接下来，通过SERS反向信号比率响应和新颖的无酶分子信号扩增，在miRNA定量分析中获得了扩增的信号和降低的黑值。更重要的是，BSR探针在细胞中显示出良好的稳定性，并成功用于从MCF-7细胞中精确追踪和定量miR-203。因此，报道的BSR探针是可靠监测活细胞中生物分子动力学的潜在工具。在miRNA定量分析中获得了放大的信号和降低的黑值。更重要的是，BSR探针在细胞中显示出良好的稳定性，并成功用于从MCF-7细胞中精确追踪和定量miR-203。因此，报告的BSR探针是可靠监测活细胞中生物分子动力学的潜在工具。在miRNA定量分析中获得了放大的信号和降低的黑值。更重要的是，BSR探针在细胞中显示出良好的稳定性，并成功用于从MCF-7细胞中精确追踪和定量miR-203。因此，报告的BSR探针是可靠监测活细胞中生物分子动力学的潜在工具。

更新日期：2021-01-26

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11